In vivo observation of protein diffusivity in rabbit lenses.

We report the in vivo observation of protein diffusivity in rabbit lenses with the use of laser light-scattering spectroscopy. Levels of laser light irradiation, which we estimate to correspond to a level less than that used in routine ophthalmoscopy, were adequate for observation. The in vivo measurements agreed with measurements on the same lens after excision from the animal. Since changes in protein diffusivity reflecting even very small degrees of protein aggregation can be observed by this method, it should make possible quantitative studies of incipient lens opacification.