Tanaka T, Ishimoto C
Invest Ophthalmol Vis Sci. 1977 Feb;16(2):135-40.
We report the in vivo observation of protein diffusivity in rabbit lenses with the use of laser light-scattering spectroscopy. Levels of laser light irradiation, which we estimate to correspond to a level less than that used in routine ophthalmoscopy, were adequate for observation. The in vivo measurements agreed with measurements on the same lens after excision from the animal. Since changes in protein diffusivity reflecting even very small degrees of protein aggregation can be observed by this method, it should make possible quantitative studies of incipient lens opacification.
我们报告了利用激光散射光谱法对兔晶状体中蛋白质扩散率进行的体内观察。我们估计激光照射水平低于常规检眼镜检查所使用的水平，该水平足以进行观察。体内测量结果与从动物身上切除后对同一晶状体进行的测量结果一致。由于通过这种方法可以观察到反映即使非常小程度蛋白质聚集的蛋白质扩散率变化，因此它应该能够对早期晶状体混浊进行定量研究。
