在光流体芯片上使用荧光相关光谱法进行超灵敏Qβ噬菌体分析。
Ultrasensitive Qbeta phage analysis using fluorescence correlation spectroscopy on an optofluidic chip.
作者信息
Rudenko M I, Kühn S, Lunt E J, Deamer D W, Hawkins A R, Schmidt H
机构信息
School of Engineering, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
出版信息
Biosens Bioelectron. 2009 Jul 15;24(11):3258-63. doi: 10.1016/j.bios.2009.04.005. Epub 2009 Apr 16.
Abstract
We demonstrate detection and analysis of the Qbeta bacteriophage on the single virus level using an integrated optofluidic biosensor. Individual Qbeta phages with masses on the order of attograms were sensed and analyzed on a silicon chip in their natural liquid environment without the need for virus immobilization. The diffusion coefficient of the viruses was extracted from the fluorescence signal by means of fluorescence correlation spectroscopy (FCS) and found to be 15.90+/-1.50 microm(2)/s in excellent agreement with previously published values. The aggregation and disintegration of the phage were also observed. Virus flow velocities determined by FCS were in the 60-300 microm/s range. This study suggests considerable potential for an inexpensive and portable sensor capable of discrimination between viruses of different sizes.
摘要
我们展示了使用集成光流体生物传感器在单病毒水平上对Qβ噬菌体进行检测和分析。在硅芯片上，在其自然液体环境中无需固定病毒，就可以检测和分析质量约为阿托克量级的单个Qβ噬菌体。通过荧光相关光谱法（FCS）从荧光信号中提取病毒的扩散系数，发现其为15.90±1.50微米²/秒，与先前发表的值非常吻合。还观察到了噬菌体的聚集和解体。由FCS测定的病毒流速在60 - 300微米/秒范围内。这项研究表明，一种能够区分不同大小病毒的廉价便携式传感器具有巨大潜力。
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