一株在5-甲基胞嘧啶处具有高频C到T突变的大肠杆菌菌株的分离与鉴定
Isolation and characterization of an Escherichia coli strain with a high frequency of C-to-T mutations at 5-methylcytosines.
作者信息
Ruiz S M, Létourneau S, Cupples C G
机构信息
Department of Biology, Concordia University, Montréal, Québec, Canada.
出版信息
J Bacteriol. 1993 Aug;175(16):4985-9. doi: 10.1128/jb.175.16.4985-4989.1993.
Abstract
We used a genetic selection system to isolate a strain of Escherichia coli with a high frequency of C-to-T transition mutations at the second C of the sequence CCAGG. Cytosines in other sequences do not mutate to thymine at a high frequency in this strain, and the frequencies of other base substitution mutations are not increased to the same extent. The gene responsible for the mutator phenotype has been mapped to 43 min on the E. coli chromosome. Several lines of evidence indicate that this gene is distinct from the very short patch repair gene vsr.
摘要
我们使用了一种基因筛选系统来分离出一株大肠杆菌，该菌株在序列CCAGG的第二个C处发生C到T转换突变的频率很高。在该菌株中，其他序列中的胞嘧啶不会高频突变为胸腺嘧啶，并且其他碱基替换突变的频率也不会增加到相同程度。导致突变体表型的基因已定位到大肠杆菌染色体上的43分钟处。几条证据表明，该基因与极短补丁修复基因vsr不同。
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