Tanimoto K, An F Y, Clewell D B
Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor 48109.
J Bacteriol. 1993 Aug;175(16):5260-4. doi: 10.1128/jb.175.16.5260-5264.1993.
pAD1, a conjugative, 60-kb, hemolysin-bacteriocin plasmid in Enterococcus faecalis, encodes a mating response to a small peptide sex pheromone, cAD1, secreted by potential recipient bacteria. A gene, traC, encoding a 60.7-kDa protein with a typical amino terminal signal peptide, was identified within a region that appears to encode a product that binds to exogenous pheromone. A cloned segment of DNA containing traC resulted in specific binding of cells to synthetic cAD1. The putative traC product has strong similarity to a product of the E. faecalis plasmid pCF10 as well as oligopeptide binding proteins of Escherichia coli, Salmonella typhimurium, and Bacillus subtilis.
pAD1是粪肠球菌中一种可接合的、大小为60 kb的溶血素-细菌素质粒，它编码对潜在受体细菌分泌的小肽性信息素cAD1的交配反应。在一个似乎编码与外源性信息素结合产物的区域内，鉴定出一个名为traC的基因，该基因编码一种具有典型氨基末端信号肽的60.7 kDa蛋白质。含有traC的一段克隆DNA导致细胞与合成的cAD1特异性结合。推测的traC产物与粪肠球菌质粒pCF10的产物以及大肠杆菌、鼠伤寒沙门氏菌和枯草芽孢杆菌的寡肽结合蛋白有很强的相似性。
