Titorenko V I, Waterham H R, Cregg J M, Harder W, Veenhuis M
Laboratory for Electron Microscopy, University of Groningen, Haren, The Netherlands.
Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7470-4. doi: 10.1073/pnas.90.16.7470.
We have studied the genetic interactions between mutant alleles in 12 genes, designated PER1-PER12, which are essential for peroxisome biogenesis in the yeast Hansenula polymorpha. Recessive mutations in any of these genes determined three different morphological phenotypes: (i) complete absence of peroxisomes (Per-); (ii) presence of small peroxisomes in conjunction with a major fraction of peroxisomal matrix proteins in the cytosol (Pim-); and (iii) presence of peroxisomes with aberrant crystalline matrix substructure (Pss-). Extensive complementation analysis showed many cases of noncomplementation--that is, diploids that contained both wild-type and mutant alleles of two different PER genes were unable to grow on methanol and showed peroxisomal defects. The observed cases of unlinked noncomplementation appeared to be gene and allele specific and were predominantly observed at lower temperatures (cold sensitive). The genetic results obtained were used to formulate a model of PER gene product interactions. In this model, five PER gene products are key or core components of the complex. Other PER gene products appear to play a more peripheral role.
我们研究了12个基因（命名为PER1 - PER12）中突变等位基因之间的遗传相互作用，这些基因对于多形汉逊酵母中过氧化物酶体的生物合成至关重要。这些基因中任何一个的隐性突变都决定了三种不同的形态学表型：（i）完全没有过氧化物酶体（Per-）；（ii）存在小的过氧化物酶体，同时大部分过氧化物酶体基质蛋白存在于细胞质中（Pim-）；（iii）存在具有异常晶体基质亚结构的过氧化物酶体（Pss-）。广泛的互补分析显示了许多非互补的情况，即包含两个不同PER基因的野生型和突变等位基因的二倍体无法在甲醇上生长，并表现出过氧化物酶体缺陷。观察到的非连锁非互补情况似乎是基因和等位基因特异性的，并且主要在较低温度下观察到（冷敏感）。所获得的遗传结果被用于构建一个PER基因产物相互作用的模型。在这个模型中，五个PER基因产物是该复合物的关键或核心成分。其他PER基因产物似乎起着更外围的作用。
