Hadwiger J A, Wittenberg C, Richardson H E, de Barros Lopes M, Reed S I
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6255-9. doi: 10.1073/pnas.86.16.6255.
Two Saccharomyces cerevisiae genes were isolated based upon their dosage-dependent rescue of a temperature-sensitive mutation of the gene CDC28, which encodes a protein kinase involved in control of cell division. CLN1 and CLN2 encode closely related proteins that also share homology with cyclins. Cyclins, characterized by a dramatic periodicity of abundance through the cell cycle, are thought to be involved in mitotic induction in animal cells. A dominant mutation in the CLN2 gene, CLN2-1, advances the G1- to S-phase transition in cycling cells and impairs the ability of cells to arrest in G1 phase in response to external signals, suggesting that the encoded protein is involved in G1 control of the cell cycle in Saccharomyces.
基于对编码参与细胞分裂控制的蛋白激酶的CDC28基因温度敏感突变的剂量依赖性拯救，分离出了两个酿酒酵母基因。CLN1和CLN2编码紧密相关的蛋白质，这些蛋白质也与细胞周期蛋白具有同源性。细胞周期蛋白的特征是在整个细胞周期中丰度具有显著的周期性，被认为参与动物细胞的有丝分裂诱导。CLN2基因中的一个显性突变CLN2-1，可促进循环细胞中从G1期到S期的转变，并损害细胞响应外部信号而停滞在G1期的能力，这表明编码的蛋白质参与酿酒酵母细胞周期的G1期控制。
