泰国豆芽中沙门氏菌的出现情况。

The occurrence of salmonellae in bean sprouts in Thailand.

作者信息

Jerngklinchan J, Saitanu K

机构信息

Division of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.

出版信息

Southeast Asian J Trop Med Public Health. 1993 Mar;24(1):114-8.

PMID:8362284

Abstract

Detection of Salmonella spp. in 344 samples of bean sprouts was attempted by pre-enriched in buffered peptone water (BPW) or lactose broth (LB) at 37 degrees C, followed by selective enrichments, selenite cystein broth (SCB) and Rappaport medium (RV) at 43 degrees C. Four selective media, bismuth sulphite agar (BSA), brilliant green agar (BGA), Salmonella-Shigella agar (SSA) and MacConkey agar (MA) were used for isolation. Salmonellae were isolated from 30 (8.7%) samples. They were serotypes lexington (56.7%), orion (16.7%), senftenberg (16.7%), tennessee (3.3%), poona (3.3%) and weltevreden (3.3%). Pre-enrichment in LB, followed by enrichment in SCB and streaking on to BSA gave the highest recoveries. Fifteen of 30 strains isolated were sensitive to all 14 antibiotics tested. Seven strains were resistant to one, and a strains showed multiple resistance to 2-4 antimicrobial agents.

摘要

尝试通过在37℃下于缓冲蛋白胨水（BPW）或乳糖肉汤（LB）中预富集，然后在43℃下进行选择性富集，即亚硒酸盐胱氨酸肉汤（SCB）和Rappaport培养基（RV），来检测344份豆芽样本中的沙门氏菌属。使用四种选择性培养基，即亚硫酸铋琼脂（BSA）、亮绿琼脂（BGA）、沙门氏菌-志贺氏菌琼脂（SSA）和麦康凯琼脂（MA）进行分离。从30份（8.7%）样本中分离出了沙门氏菌。它们的血清型为列克星敦（56.7%）、猎户座（16.7%）、森夫滕贝格（16.7%）、田纳西（3.3%）、浦那（3.3%）和韦尔泰夫登（3.3%）。在LB中预富集，随后在SCB中富集并划线接种到BSA上，回收率最高。分离出的30株菌株中有15株对所测试的全部14种抗生素敏感。7株对一种抗生素耐药，1株对2 - 4种抗菌药物表现出多重耐药。