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花椰菜芽中质体淀粉磷酸转运体的鉴定

Identification of the putative hexose-phosphate translocator of amyloplasts from cauliflower buds.

作者信息

Batz O, Scheibe R, Neuhaus H E

机构信息

Fachbereich Biologie/Chemie, Universität Osnabrück, Federal Republic of Germany.

出版信息

Biochem J. 1993 Aug 15;294 ( Pt 1)(Pt 1):15-7. doi: 10.1042/bj2940015.

DOI:10.1042/bj2940015
PMID:8363565
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1134558/
Abstract

Starch synthesis in amyloplasts isolated from cauliflower buds is strongly inhibited by the addition of micromolar concentrations of 4,4'-di-isothiocyano-2,2'-stilbenedisulphonic acid (DIDS). Using [3H]DIDS it was possible to label specifically a 31.6 kDa membrane protein of the envelope fraction of isolated amyloplasts. The intensity of the radioactive label was decreased in the presence of glucose 6-phosphate or dihydroxyacetone phosphate, indicating that this protein might be the amyloplastic hexosephosphate translocator.

摘要

从菜花芽中分离出的造粉体中的淀粉合成，会被添加微摩尔浓度的4,4'-二异硫氰酸根合-2,2'-二苯乙烯二磺酸（DIDS）强烈抑制。使用[³H]DIDS能够特异性标记分离出的造粉体包膜部分的一种31.6 kDa膜蛋白。在存在6-磷酸葡萄糖或磷酸二羟丙酮的情况下，放射性标记的强度降低，这表明该蛋白可能是造粉体己糖磷酸转运体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4f/1134558/718bc43c4512/biochemj00105-0026-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4f/1134558/96956ecbbe7b/biochemj00105-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4f/1134558/718bc43c4512/biochemj00105-0026-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4f/1134558/96956ecbbe7b/biochemj00105-0026-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa4f/1134558/718bc43c4512/biochemj00105-0026-b.jpg

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ADP-glucose drives starch synthesis in isolated maize endosperm amyloplasts: characterization of starch synthesis and transport properties across the amyloplast envelope.

本文引用的文献

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2
Specific Labeling of the Phosphate Translocator in C(3) and C(4) Mesophyll Chloroplasts by Tritiated Dihydro-DIDS (1,2-Ditritio-1,2-[2,2' -Disulfo-4,4' -Diisothiocyano] Diphenylethane).三氚代二氢二异硫代二苯腙（1,2-二硫代-1,2-[2,2' -二磺酰基-4,4' -二异硫氰基]二苯乙烷）对 C(3)和 C(4) 叶肉叶绿体中磷酸盐转运蛋白的特异性标记。
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