Roeling T A, Kruk M R, Schuurmans R, Veening J G
Department of Anatomy and Embryology, Faculty of Medical Sciences, Catholic University of Nijmegen, The Netherlands.
Brain Res. 1993 Jun 25;615(1):121-7. doi: 10.1016/0006-8993(93)91122-9.
Several studies, using electrical stimulation of parts of the hypothalamus, have shown, that different parts of the hypothalamus yield different behavioural responses upon stimulation. In order to differentiate between stimulation of neuronal cell bodies and passing fibres and to investigate the role of GABA in hypothalamically elicited behaviour, 25 local injections with bicucculline methiodide, a GABA antagonist, (35 ng/0.2 microliter) were performed in the ventral parts of the hypothalamus of 16 freely moving rats in a social environment. A cannula system was used that allowed injection without interruption of the ongoing social interactions. Digging, gnawing, drinking and attack behaviour were elicited in different animals. By plotting the behavioural responses of the animals into a detailed hypothalamic atlas, we assessed the hypothalamic distribution of the elicited behavioural responses. A number of injections elicited a combination of two or three different responses, probably due to diffusion of the substance, thus disinhibiting more than one behavioural system. Our results are in general agreement with previous electrical stimulation data and show that, in an overlapping pattern, different populations of neurons are involved in the elicitation of digging, gnawing, drinking and attack behaviour. In the hypothalamus, a tonic GABAergic inhibition of neurons involved in the display of these types of behaviour appears to exist.
几项利用电刺激下丘脑不同部位的研究表明，下丘脑的不同部位在受到刺激时会产生不同的行为反应。为了区分对神经元细胞体和传导纤维的刺激，并研究γ-氨基丁酸（GABA）在下丘脑引发的行为中的作用，对16只在社交环境中自由活动的大鼠的下丘脑腹侧进行了25次局部注射甲基荷包牡丹碱（一种GABA拮抗剂，35纳克/0.2微升）。使用了一种插管系统，该系统允许在不中断正在进行的社交互动的情况下进行注射。在不同的动物中引发了挖掘、啃咬、饮水和攻击行为。通过将动物的行为反应绘制到详细的下丘脑图谱中，我们评估了引发的行为反应在下丘脑的分布情况。许多注射引发了两种或三种不同反应的组合，这可能是由于物质的扩散，从而使不止一个行为系统的抑制作用解除。我们的结果总体上与之前的电刺激数据一致，表明以重叠的模式，不同的神经元群体参与了挖掘、啃咬、饮水和攻击行为的引发。在下丘脑中，似乎存在对参与这些类型行为表现的神经元的持续性GABA能抑制作用。
