Dunn J T, Crutchfield H E, Gutekunst R, Dunn A D
Department of Medicine, University of Virginia School of Medicine, Charlottesville.
Thyroid. 1993 Summer;3(2):119-23. doi: 10.1089/thy.1993.3.119.
Urinary iodine excretion is currently the most convenient laboratory marker of iodine deficiency. Accelerating international interest in correcting this condition demands rapid, simple methods for assessment and monitoring. We describe two adaptations of the Sandell-Kolthoff reaction, in which urine is first digested with chloric acid and iodine then determined from its catalytic reduction of ceric ammonium sulfate in the presence of arsenious acid. Both methods use gentle digestion by chloric acid in a heating block. Method A detects iodine in a colorimeter, method B by the indicator ferroin and a stopwatch. Results with 12 samples ranging from 1.8 to 19.0 micrograms/dL (0.14-1.48 mumol/L) differed from those in a reference laboratory by a mean of 9.1% for method A and 15.7% for method B. One technician can perform at least 150 tests per day at a total cost of less than $0.50 each. The speed, low cost, and simple instrumentation make these methods well suited to epidemiological assessment of iodine deficiency in developing countries.
尿碘排泄是目前评估碘缺乏最便捷的实验室指标。国际社会对纠正这一状况的关注度不断提高，需要快速、简便的评估和监测方法。我们描述了桑德尔-科尔托夫反应的两种改进方法，即先用氯酸消化尿液，然后在亚砷酸存在的情况下，通过碘对硫酸铈铵的催化还原作用来测定碘。两种方法均采用在加热块中用氯酸进行温和消化。方法A用比色计检测碘，方法B用邻二氮菲指示剂和秒表检测。对12份碘含量在1.8至19.0微克/分升（0.14 - 1.48微摩尔/升）之间的样本进行检测，方法A的结果与参考实验室的结果相比，平均差异为9.1%，方法B为15.7%。一名技术人员每天至少可进行150次检测，每次检测总成本不到0.50美元。这些方法速度快、成本低且仪器简单，非常适合发展中国家碘缺乏的流行病学评估。
