对溴苯甲酰溴（一种磷脂酶A2抑制剂）对豚鼠子宫体外花生四烯酸释放和前列腺素合成的影响。
Effect of p-bromophenacyl bromide, an inhibitor of phospholipase A2, on arachidonic acid release and prostaglandin synthesis by the guinea-pig uterus in vitro.
作者信息
Mitchell S, Poyser N L, Wilson N H
出版信息
Br J Pharmacol. 1977 Jan;59(1):107-13. doi: 10.1111/j.1476-5381.1977.tb06983.x.
Abstract
The synthesis of prostaglandins F2alpha and E2 by guinea-pig uterine homogenates was inhibited by p-bromophenacyl bromide (PBPAB), an inhibitor of phospholipase A2. 2 Metabolism of prostaglandin F2alpha by uterine homogenates was undetectable; this was not affected by PBPAB. 3 There was no significant difference between the amounts of arachidonic acid released from uterine homogenates on days 7 and 1k of the oestrous cycle. Small amounts of dihomo-gamma-linolenic acid were detected in the homogenates. 4 The release of arachidonic acid from uterine homogenates was greatly inhibited by PBPAB. 5 Addition of exogenous arachidonic acid to uterine homogenates did not overcome the inhibition of uterine prostaglandin F2alpha synthesis produced by PBPAB. 6 It is concluded that PBPAB inhibits both the release of arachidonic acid from the guinea-pig uterus and its subsequent conversion into prostaglandins.
摘要
磷脂酶A2的抑制剂对溴苯甲酰溴（PBPAB）可抑制豚鼠子宫匀浆合成前列腺素F2α和E2。2子宫匀浆对前列腺素F2α的代谢无法检测到；这不受PBPAB影响。3发情周期第7天和第11天从子宫匀浆释放的花生四烯酸量之间没有显著差异。在匀浆中检测到少量二高-γ-亚麻酸。4 PBPAB可极大地抑制子宫匀浆中花生四烯酸的释放。5向子宫匀浆中添加外源性花生四烯酸并不能克服PBPAB对子宫前列腺素F2α合成的抑制作用。6得出结论，PBPAB既抑制豚鼠子宫中花生四烯酸的释放，也抑制其随后转化为前列腺素。
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