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利用一种简单的裂解方法和聚合酶链反应检测临床样本中的结核分枝杆菌DNA。

Detection of Mycobacterium tuberculosis DNA in clinical samples by using a simple lysis method and polymerase chain reaction.

作者信息

Folgueira L, Delgado R, Palenque E, Noriega A R

机构信息

Department of Microbiology, Hospital Doce de Octubre, Madrid, Spain.

出版信息

J Clin Microbiol. 1993 Apr;31(4):1019-21. doi: 10.1128/jcm.31.4.1019-1021.1993.

DOI:10.1128/jcm.31.4.1019-1021.1993
PMID:8463383
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263610/
Abstract

We have evaluated the polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples from patients with tuberculous infection. Two simple methods for mycobacterial DNA release have been compared: sonication and lysis with nonionic detergents and proteinase K. The more effective method was the enzymatic technique. By using this protocol with 75 specimens we detected M. tuberculosis DNA in all of the samples, whereas only 48 and 71 samples were positive by acid-fast staining and culture, respectively.

摘要

我们评估了聚合酶链反应在检测结核感染患者临床样本中结核分枝杆菌的应用。比较了两种简单的分枝杆菌DNA释放方法：超声处理以及用非离子去污剂和蛋白酶K裂解。更有效的方法是酶法。采用该方案对75份标本进行检测，我们在所有样本中均检测到结核分枝杆菌DNA，而抗酸染色和培养分别仅48份和71份样本呈阳性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6551/263610/7cbd4053b706/jcm00016-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6551/263610/7cbd4053b706/jcm00016-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6551/263610/7cbd4053b706/jcm00016-0268-a.jpg

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