Cellona R V, Walsh G P, Fajardo T T, Abalos R M, dela Cruz E C, Guido-Villahermosa L, Felicio-Balagon M V, Steenbergen G J, Douglas J T
Clinical Research Branch, Leonard Wood Memorial Center for Leprosy Research, Cebu City, The Phillippines.
Int J Lepr Other Mycobact Dis. 1993 Jun;61(2):192-8.
An indirect enzyme-linked immunosorbent assay (ELISA) using natural disaccharide octyl bovine serum albumin (ND-O-BSA) as antigen was used in testing leprosy patients, contacts and a normal population in Cebu, The Philippines, from 1985 to 1989. A total of 1413 persons were studied. The results suggested that ELISA reactivity and the bacterial index (BI) correlate in a general way. In multibacillary (MB) leprosy, positivity ranges from 54.2% to 92.3% among patients with a BI of < 2+ to > 4+ on the Ridley scale, with an overall average of 84.5%. Paucibacillary (PB) leprosy patients have a low degree of reactivity, with only 15.0% ELISA positive. The test is more efficient in detecting MB than PB leprosy. The contacts of MB leprosy showed 6.5% positivity; contacts of PB leprosy, 7.0% positivity. The normal population showed 1.7% positive ELISA or 17 per thousand population, which is very much less than that of the household contacts. However, because the normal population is a much larger population than the household contact population in a community, more new leprosy cases would emanate from it. Leprosy workers are concerned about the transmission of the disease to household contacts. However, for the reason stated above, we should be more concerned with the silent spread of the disease to the normal population in the community.(ABSTRACT TRUNCATED AT 250 WORDS)
1985年至1989年期间，在菲律宾宿务，采用以天然二糖辛基牛血清白蛋白（ND - O - BSA）为抗原的间接酶联免疫吸附测定（ELISA）法，对麻风病人、接触者及正常人群进行检测。共研究了1413人。结果表明，ELISA反应性与细菌指数（BI）总体上存在相关性。在多菌型（MB）麻风病中，按照里德利分级标准，BI为<2 +至>4 +的患者中，阳性率范围为54.2%至92.3%，总体平均为84.5%。少菌型（PB）麻风病患者的反应性较低，ELISA阳性率仅为15.0%。该检测在检测MB麻风病方面比PB麻风病更有效。MB麻风病接触者的阳性率为6.5%；PB麻风病接触者的阳性率为7.0%。正常人群的ELISA阳性率为1.7%，即每千人口中有17例阳性，这远低于家庭接触者的阳性率。然而，由于社区中的正常人群数量比家庭接触人群大得多，因此会从中出现更多新的麻风病病例。麻风病防治工作者关注疾病向家庭接触者的传播。然而，基于上述原因，我们应更关注疾病在社区中向正常人群的隐匿传播。（摘要截选于250字）
