采用柱切换紫外检测的高效液相色谱法灵敏测定人血浆和尿液中新型红霉素衍生物EM523。

Sensitive high-performance liquid chromatographic determination of EM523, a new erythromycin derivative, in human plasma and urine with ultraviolet detection using column switching.

作者信息

Yamashita K, Motohashi M, Yashiki T

机构信息

Takeda Analytical Research Laboratories, Ltd., Osaka, Japan.

出版信息

J Chromatogr. 1993 Jun 23;616(1):144-50. doi: 10.1016/0378-4347(93)80481-i.

DOI:10.1016/0378-4347(93)80481-i

PMID:8376485

Abstract

A sensitive high-performance liquid chromatographic (HPLC) method using column switching is described for the determination of EM523 (I), a new erythromycin derivative, in human plasma and urine. The analyte was extracted from alkalinized plasma or urine with a mixture of n-hexane and acetone. After the evaporation of the organic layer, the reconstituted residue was injected into the HPLC system and separated on the first column. After column switching, the heart-cut fraction contamination the analyte was further separated on the second column and monitored by ultraviolet absorbance at 210 nm. The detection limits were 1 ng/ml in plasma and 10 ng/ml in urine. The method was applied to the clinical trials of I.

摘要

描述了一种使用柱切换的灵敏高效液相色谱（HPLC）方法，用于测定人血浆和尿液中的新型红霉素衍生物EM523（I）。分析物用正己烷和丙酮的混合物从碱化的血浆或尿液中萃取。有机层蒸发后，将重构的残渣注入HPLC系统，并在第一根柱上进行分离。柱切换后，含有分析物的中心切割馏分在第二根柱上进一步分离，并通过210nm处的紫外吸光度进行监测。血浆中的检测限为1ng/ml，尿液中的检测限为10ng/ml。该方法应用于I的临床试验。