Yamashita K, Motohashi M, Yashiki T
Takeda Analytical Research Laboratories, Ltd., Osaka, Japan.
J Chromatogr. 1993 Jun 23;616(1):144-50. doi: 10.1016/0378-4347(93)80481-i.
A sensitive high-performance liquid chromatographic (HPLC) method using column switching is described for the determination of EM523 (I), a new erythromycin derivative, in human plasma and urine. The analyte was extracted from alkalinized plasma or urine with a mixture of n-hexane and acetone. After the evaporation of the organic layer, the reconstituted residue was injected into the HPLC system and separated on the first column. After column switching, the heart-cut fraction contamination the analyte was further separated on the second column and monitored by ultraviolet absorbance at 210 nm. The detection limits were 1 ng/ml in plasma and 10 ng/ml in urine. The method was applied to the clinical trials of I.
描述了一种使用柱切换的灵敏高效液相色谱（HPLC）方法，用于测定人血浆和尿液中的新型红霉素衍生物EM523（I）。分析物用正己烷和丙酮的混合物从碱化的血浆或尿液中萃取。有机层蒸发后，将重构的残渣注入HPLC系统，并在第一根柱上进行分离。柱切换后，含有分析物的中心切割馏分在第二根柱上进一步分离，并通过210nm处的紫外吸光度进行监测。血浆中的检测限为1ng/ml，尿液中的检测限为10ng/ml。该方法应用于I的临床试验。
