Clastogenicity of chlorambucil to the mouse bone marrow: consideration in relation to its genetic specificity of action in some assays.

The human carcinogen and mouse germ cell mutagen chlorambucil is reported here to give a strong positive response in the mouse bone marrow micronucleus assay (4 mg/kg; single intraperitoneal injection). An increased incidence of micronucleated bone marrow white cells was also observed. It is concluded that chlorambucil is a broad spectrum mutagen that possesses subtle and specific mutagenic activities in some assay systems.