Roider J, Hillenkamp F, Flotte T, Birngruber R
Wellman Laboratories of Photomedicine, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8643-7. doi: 10.1073/pnas.90.18.8643.
Repetitive exposure to short laser pulses is shown to cause selective damage to absorbing structures (cells, organelles, or enzymes) with pulse energies below the threshold energy for single-pulse damage. Directly adjacent structures are spared in vivo. Additivity of (presumably nonphotochemical) subthreshold effects is demonstrated. Selective damage to the retinal pigment epithelium with sparing of the neural retina is shown (514 nm, 5 microseconds, 1-500 pulses at 500 Hz, 2- to 10-microJ pulse energy). A melanin granule model has been developed and applied to the experimental situation. Histological results as well as the basic mechanism for these effects are discussed.
研究表明，重复暴露于短激光脉冲会对吸收结构（细胞、细胞器或酶）造成选择性损伤，其脉冲能量低于单脉冲损伤的阈值能量。在体内，直接相邻的结构未受影响。研究证明了（可能是非光化学的）亚阈值效应具有可加性。研究显示，对视网膜色素上皮有选择性损伤，而神经视网膜未受影响（514纳米，5微秒，500赫兹下1 - 500个脉冲，脉冲能量为2至10微焦）。已建立了黑色素颗粒模型并应用于实验情况。文中讨论了组织学结果以及这些效应的基本机制。
