Lundkvist A, Scholander C, Niklasson B
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Arch Virol. 1993;132(3-4):255-65. doi: 10.1007/BF01309537.
Rabbit anti-idiotypic antibodies (anti-ids) were generated against three bank vole and one human monoclonal antibody (MAb) specific for the two envelope glycoproteins of Puumala virus (G1 and G2). The anti-ids were purified by sequential immunoaffinity chromatography. Each anti-id inhibited the antigen binding of its respective MAb in a competitive ELISA. This inhibition, and the absence of cross-reactivity among the anti-ids for heterologous MAbs, showed that they all were specific for unique determinants on the antigen binding site of the homologous MAb. The anti-ids reacted with non-infected Vero E6 cells when examined by immunofluorescence and ELISA, indicating the presence of antibodies that mimic epitopes on the virus. Preincubation of Vero E6 cells with two of the anti-ids produced against neutralizing MAbs inhibited Puumala virus infection, suggesting that these two anti-ids blocked a cellular component involved in virus infection.
针对三种棕背䶄和一种针对普马拉病毒两种包膜糖蛋白（G1和G2）的人单克隆抗体（MAb），制备了兔抗独特型抗体（抗独特型抗体）。通过连续免疫亲和层析纯化抗独特型抗体。在竞争性酶联免疫吸附测定（ELISA）中，每种抗独特型抗体均抑制其相应单克隆抗体的抗原结合。这种抑制作用以及抗独特型抗体对异源单克隆抗体无交叉反应性，表明它们均对同源单克隆抗体抗原结合位点上的独特决定簇具有特异性。通过免疫荧光和ELISA检测时，抗独特型抗体与未感染的非洲绿猴肾细胞（Vero E6）发生反应，表明存在模拟病毒表位的抗体。用针对中和性单克隆抗体产生的两种抗独特型抗体对Vero E6细胞进行预孵育，可抑制普马拉病毒感染，这表明这两种抗独特型抗体阻断了参与病毒感染的细胞成分。
