在小鼠模型中，肠道对异源轮状病毒感染的抗体反应持续超过1年。
Persistence of intestinal antibody response to heterologous rotavirus infection in a murine model beyond 1 year.
作者信息
Shaw R D, Merchant A A, Groene W S, Cheng E H
机构信息
Department of Medicine, Northport Veterans Affairs Medical Center, New York 11768.
出版信息
J Clin Microbiol. 1993 Feb;31(2):188-91. doi: 10.1128/jcm.31.2.188-191.1993.
Abstract
We used an ELISPOT (enzyme-linked immunosorbent spot) assay to quantitate the long-term rotavirus-specific intestinal antibody response in a murine model. The frequency of murine intestinal antibody-secreting cells (ASCs) was followed for a period of 1 year after a single dose of rhesus rotavirus (10(6) PFU) was administered at 10 days of age. Some animals were boosted at that time with a second dose. One year after infection, virus-specific ASCs declined from acute-phase levels, but they were still present at significant levels (1.32 x 10(4) virus-specific ASCs per 10(6) intestinal mononuclear cells; approximately 17% of the previously reported response at 1 month after infection). A booster dose 1 year after the primary infection produced a 100% increase in virus-specific ASCs but did not restore the response to that of the primary infection.
摘要
我们使用酶联免疫斑点（ELISPOT）测定法在小鼠模型中定量长期轮状病毒特异性肠道抗体反应。在10日龄时给予单剂量恒河猴轮状病毒（10⁶ PFU）后，跟踪小鼠肠道抗体分泌细胞（ASC）的频率长达1年。当时一些动物用第二剂进行加强免疫。感染1年后，病毒特异性ASC从急性期水平下降，但仍以显著水平存在（每10⁶肠道单核细胞中有1.32×10⁴病毒特异性ASC；约为感染后1个月时先前报道反应的17%）。初次感染1年后的加强剂量使病毒特异性ASC增加了100%，但未将反应恢复到初次感染时的水平。
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