对清醒和麻醉大鼠气管黏液纤毛清除功能的测量。

Measurement of mucociliary clearance from the trachea of conscious and anesthetized rats.

作者信息

Patrick G, Stirling C

出版信息

J Appl Physiol Respir Environ Exerc Physiol. 1977 Mar;42(3):451-5. doi: 10.1152/jappl.1977.42.3.451.

DOI:10.1152/jappl.1977.42.3.451

Abstract

A technique is described whereby particles are injected as a suspension into the trachea of conscious rats. Tracheostomy and cannulation are performed under halothane, but the particles are injected 10 min after recovery. Clearance of BaSO4 particles labeled with 133Ba was determined with a collimated slit gamma-counter, either aligned with the region of particle deposition or used to scan along the animal. Compared with clearance up the trachea in conscous rats, the effect of halothane, thiopental and pentobarbital was to inhibit clearance. With halothane, clearance was resumed at the control rate 0-3 min after anesthesia lasting 7-9 min.

摘要

本文描述了一种将颗粒作为悬浮液注入清醒大鼠气管的技术。气管切开术和插管在氟烷麻醉下进行，但颗粒在恢复10分钟后注入。用准直狭缝γ计数器测定用133Ba标记的硫酸钡颗粒的清除率，该计数器可与颗粒沉积区域对齐或用于沿动物扫描。与清醒大鼠气管内的清除率相比，氟烷、硫喷妥钠和戊巴比妥的作用是抑制清除率。使用氟烷时，在持续7-9分钟的麻醉后0-3分钟，清除率恢复到对照水平。