白色念珠菌的倍性测定
Ploidy determination of Canadida albicans.
作者信息
Olaiya A F, Sogin S J
出版信息
J Bacteriol. 1979 Dec;140(3):1043-9. doi: 10.1128/jb.140.3.1043-1049.1979.
Abstract
The dimorphic yeast Candida albicans, as a member of the fungi imperfecti, has been assumed to be in the haploid, or imperfect, state. The deoxyribonucleic acid content of this species has been measured by flow microfluorometry, a technique capable of analyzing single cells. These results were compared with flow microfluorometric deoxyribonucleic acid determinations on a series of strains of Saccharomyces cerevisiae of known ploidy (haploid, diploid, triploid, and tetraploid). These ploidy levels were readily distinguished by the flow microfluorometry procedure. By this criterion, C. albicans was found to contain a diploid amount of deoxyribonucleic acid. Ultraviolet radiation survival and chemical mutagenesis experiments support the conclusion that both clinically isolated and laboratory strains of C. albicans are diploid.
摘要
二态性酵母白色念珠菌作为半知菌的一员，一直被认为处于单倍体或不完善状态。该物种的脱氧核糖核酸含量已通过流式细胞荧光测定法进行测量，这是一种能够分析单个细胞的技术。这些结果与对一系列已知倍性（单倍体、二倍体、三倍体和四倍体）的酿酒酵母菌株进行的流式细胞荧光测定脱氧核糖核酸的结果进行了比较。通过流式细胞荧光测定法很容易区分这些倍性水平。根据这一标准，发现白色念珠菌含有二倍体数量的脱氧核糖核酸。紫外线辐射存活和化学诱变实验支持了白色念珠菌的临床分离株和实验室菌株均为二倍体这一结论。
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