Arai T, Watanabe M, Onodera M, Yamashita T, Masunaga A, Itoyama S, Itoh K, Sugawara I
Department of Pathology, Saitama Medical Center, Saitama Medical School, Japan.
Am J Pathol. 1993 Jun;142(6):1938-44.
The nm 23 gene product, which possesses nucleoside diphosphate kinase activity, is a possible mediator of cancer cell invasion and metastasis. It has been divided into two distinct gene products, nm23-H1 and nm23-H2. We have developed a method for detecting nm 23-H1 and nm 23-H2 RNA using the polymerase chain reaction, based on the amplification of complementary DNA copies of nm 23-H1 and nm 23-H2 RNA. Using this method, the nm 23-H1 and nm 23-H2 messenger (m)RNA levels in 35 thyroid papillary carcinomas, 11 metastatic lymph nodes from patients with thyroid papillary carcinomas, five thyroid follicular adenomas, and three normal thyroid tissue samples were studied. Both nm 23-H1 and nm 23-H2 mRNA were expressed ubiquitously in normal and malignant thyroid tissues. However, in metastatic lymph nodes no (3 of 11) or weak expression (8 of 11) of nm 23-H1 mRNA was observed, the extent of which was inversely proportional to the degree of cancer cell occupancy, whereas nm 23-H2 mRNA was expressed and the levels were similar to those in other tissue tested. These results show that nm 23-H1 only may play a role in cancer cell invasion and metastasis although the exact mechanisms involved have yet to be elucidated.
具有核苷二磷酸激酶活性的nm 23基因产物可能是癌细胞侵袭和转移的介质。它已被分为两种不同的基因产物，即nm23-H1和nm23-H2。我们基于nm 23-H1和nm 23-H2 RNA互补DNA拷贝的扩增，开发了一种利用聚合酶链反应检测nm 23-H1和nm 23-H2 RNA的方法。使用该方法，研究了35例甲状腺乳头状癌、11例甲状腺乳头状癌患者的转移淋巴结、5例甲状腺滤泡性腺瘤和3例正常甲状腺组织样本中的nm 23-H1和nm 23-H2信使（m）RNA水平。nm 23-H1和nm 23-H2 mRNA在正常和恶性甲状腺组织中均普遍表达。然而，在转移淋巴结中，未观察到nm 23-H1 mRNA表达（11例中的3例）或弱表达（11例中的8例），其程度与癌细胞占有率呈负相关，而nm 23-H2 mRNA有表达且水平与其他检测组织中的相似。这些结果表明，尽管具体机制尚待阐明，但可能只有nm 23-H1在癌细胞侵袭和转移中发挥作用。
