Blokland A, Bothmer J, Honig W, Jolles J
Department of Neuropsychology and Psychobiology, University of Limburg, Maastricht, Netherlands.
Eur J Pharmacol. 1993 Apr 28;235(2-3):275-81. doi: 10.1016/0014-2999(93)90146-9.
In the present study we evaluated a new method to assess the behavioural and biochemical effects of a brief period of acute hypoxia in the brain. In this method, cyanide is injected into the lateral ventricles. Spatial navigation performance in a Morris task was found to be impaired 1 and 5 min after an i.c.v. injection of 5.0 micrograms cyanide but not after 2.5 micrograms cyanide. Increased rate of phosphatidic acid formation, reflecting increased phospholipase C activity, were observed after injection of 5.0 micrograms cyanide, indicating that energy-dependent phosphoinositide metabolism was affected. Chronic treatment with acetyl-l-carnitine attenuated the cyanide-induced behavioural deficit, but had no effect on energy-dependent phosphoinositide metabolism. The results suggest that, in this model, acetyl-l-carnitine may act via free fatty acid metabolism, by increasing the reservoir of activated acyl groups which are involved in the reacylation of membrane phospholipids.
在本研究中，我们评估了一种新方法，以评估大脑中短暂急性缺氧的行为和生化效应。在该方法中，将氰化物注入侧脑室。在脑室内注射5.0微克氰化物后1分钟和5分钟时，发现莫里斯任务中的空间导航性能受损，但注射2.5微克氰化物后未受损。注射5.0微克氰化物后，观察到反映磷脂酶C活性增加的磷脂酸形成速率增加，表明能量依赖性磷酸肌醇代谢受到影响。用乙酰左旋肉碱进行慢性治疗可减轻氰化物诱导的行为缺陷，但对能量依赖性磷酸肌醇代谢没有影响。结果表明，在该模型中，乙酰左旋肉碱可能通过游离脂肪酸代谢起作用，即增加参与膜磷脂再酰化的活化酰基储备。
