Blackett N M, Parry D M
J Histochem Cytochem. 1977 Mar;25(3):206-14. doi: 10.1177/25.3.839062.
An improved method for the analysis of high resolution electron microscope autoradiographs is described which is simple and quick to perform. The method uses a transparent screen superimposed over the autoradiograph prints to provide information on the distribution of grains over neighboring structures, produced by radioactive disintegrations occurring in different regions of the section. This "cross-scatter" information is used to estimate the radioactivity in different structures without the need to make any assumptions about the size, shape and arrangement of the structures within the autoradiographs being analyzed. The method allows activity in membranes and other linear structures to be determined and also the accuracy attributable to the activity values obtained.
本文描述了一种用于分析高分辨率电子显微镜放射自显影片的改进方法，该方法操作简单且快速。该方法使用一个透明屏幕叠加在放射自显影片上，以提供有关由切片不同区域发生的放射性衰变产生的颗粒在相邻结构上分布的信息。这种“交叉散射”信息用于估计不同结构中的放射性，而无需对所分析的放射自显影片内结构的大小、形状和排列做出任何假设。该方法能够确定膜和其他线性结构中的活性，以及所获得活性值的准确性。
