在源自两例高分化鼻咽癌的癌细胞系中检测人乳头瘤病毒16型DNA序列的低水平表达
Detection of low level of human papilloma virus type 16 DNA sequences in cancer cell lines derived from two well-differentiated nasopharyngeal cancers.
作者信息
Huang E S, Gutsch D, Tzung K W, Lin C T
机构信息
Institute of Biomedical Sciences, Academia Sinica, Taipei.
出版信息
J Med Virol. 1993 Jul;40(3):244-50. doi: 10.1002/jmv.1890400314.
PMID:8394875
Abstract
Human papilloma virus type 16 (HPV16) related sequences were detected in two EBV-negative nasopharyngeal carcinoma (NPC) cell lines derived from two well-differentiated NPC by polymerase chain reaction. E2 and E6 related sequences of HPV16 were demonstrated using two pairs of primers derived from these two regions. DNA sequence analysis of amplified products excluded the possibility of laboratory viral DNA contamination.
摘要
通过聚合酶链反应在源自两例高分化鼻咽癌的两个EB病毒阴性鼻咽癌（NPC）细胞系中检测到16型人乳头瘤病毒（HPV16）相关序列。使用源自这两个区域的两对引物证实了HPV16的E2和E6相关序列。扩增产物的DNA序列分析排除了实验室病毒DNA污染的可能性。
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