Tung Y C, Lin K H, Chu P Y, Hsu C C, Kuo W R
Department of Clinical Laboratory, Kaohsiung Medical College, Kaohsiung, Taiwan, Republic of China.
Kaohsiung J Med Sci. 1999 May;15(5):256-62.
Nasopharyngeal carcinoma (NPC) is an unique epithelial malignancy which occurs at a high frequency in certain regions of Southeast Asia. Human papilloma virus (HPV) and Epstein-Barr virus (EBV) have both been identified in tissue specimens from NPC. Nevertheless, the association between viral infection and NPC remains unclear. The purpose of this study was to demonstrate that simultaneous infection with EBV and HPV can occur in NPC. Eighty-eight fresh tissue samples which contained sufficient and adequate DNA from patients with histologically confirmed NPC were examined for the existence of HPV and EBV DNA by polymerase chain reaction (PCR). The results showed that HPV and EBV DNA were detected in 51% and in 83% of the specimens, respectively. Coexistence of EBV and HPV in NPC was found in 42% of the samples. The "high risk" types including HPV-16 and HPV-18 accounted for 67% of 45 HPV positive samples. Furthermore, 80% of HPV-16 or HPV-18 positive samples also contained EBV DNA. Our findings suggest that coexistence of EBV and "high risk" HPV-16 or HPV-18 infection may be an important factor in the pathogenesis of NPC.
鼻咽癌（NPC）是一种独特的上皮性恶性肿瘤，在东南亚某些地区高发。人乳头瘤病毒（HPV）和EB病毒（EBV）均已在鼻咽癌组织标本中被鉴定出来。然而，病毒感染与鼻咽癌之间的关联仍不清楚。本研究的目的是证明鼻咽癌中可同时发生EBV和HPV感染。通过聚合酶链反应（PCR）对88份来自组织学确诊为鼻咽癌患者的新鲜组织样本进行检测，这些样本含有足够且合适的DNA，以检测HPV和EBV DNA的存在。结果显示，分别在51%和83%的标本中检测到HPV和EBV DNA。在42%的样本中发现鼻咽癌中EBV和HPV共存。包括HPV-16和HPV-18在内的“高危”型占45份HPV阳性样本的67%。此外，80%的HPV-16或HPV-18阳性样本也含有EBV DNA。我们的研究结果表明，EBV与“高危”HPV-16或HPV-18感染共存可能是鼻咽癌发病机制中的一个重要因素。
