Giannoudis A, Ergazaki M, Segas J, Giotakis J, Adamopoulos G, Gorgoulis V, Spandidos D A
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Cancer Lett. 1995 Mar 2;89(2):177-81. doi: 10.1016/0304-3835(94)03667-8.
We used the PCR technique to detect the Epstein-Barr virus (EBV) and human papillomavirus (HPV) DNA in paraffin-embedded tissues from Greek patients with nasopharyngeal carcinoma (NPC). The oligonucleotide primers used for the detection of EBV amplify a 375-bp long sequence from the EcoRI B fragment of the viral genome, whereas for HPV the primers amplify a 151-bp long sequence of the viral genome. The PCR products were analysed by agarose gel electrophoresis and visualised by UV illumination after staining with ethidium bromide. Sixty-three specimens were examined. EBV specific sequence was amplified in 20 (32%) and HPV in 12 (19%) out of the 63 samples. There was no co-infection with EBV and HPV. Although there is a high correlation of EBV infection with poorly differentiated NPC in patients from Southern China and South-East Asia, the restricted distribution suggests genetic or environmental cofactors in the development of the neoplasm. Our results confirm this suggestion since there was only a 32% correlation of EBV with NPC in Greece. HPV may also be involved in the carcinogenesis of EBV-negative squamous cell nasopharyngeal carcinomas.
我们运用聚合酶链反应（PCR）技术，检测了来自希腊鼻咽癌（NPC）患者石蜡包埋组织中的爱泼斯坦-巴尔病毒（EBV）和人乳头瘤病毒（HPV）DNA。用于检测EBV的寡核苷酸引物，可从病毒基因组的EcoRI B片段扩增出一段375bp长的序列，而用于HPV的引物则可扩增出一段151bp长的病毒基因组序列。PCR产物通过琼脂糖凝胶电泳进行分析，并用溴化乙锭染色后经紫外线照射显影。共检测了63个标本。63个样本中，有20个（32%）扩增出了EBV特异性序列，12个（19%）扩增出了HPV特异性序列。未发现EBV和HPV的合并感染。尽管在中国南方和东南亚患者中，EBV感染与低分化NPC高度相关，但分布受限表明肿瘤发生过程中存在遗传或环境协同因素。我们的结果证实了这一推测，因为在希腊，EBV与NPC的相关性仅为32%。HPV也可能参与EBV阴性的鳞状细胞鼻咽癌的致癌过程。
