Tyan Y S, Liu S T, Ong W R, Chen M L, Shu C H, Chang Y S
Department of Microbiology and Immunology, Chang-Gung Medical College, Kwei-shan, Taoyuan, Republic of China.
J Clin Microbiol. 1993 Jan;31(1):53-6. doi: 10.1128/jcm.31.1.53-56.1993.
The presence of Epstein-Barr virus (EBV) DNA and human papillomavirus (HPV) DNA in 74 head and neck tumor tissues was examined by the polymerase chain reaction and DNA sequencing analysis. EBV DNA sequence was detected in all 30 nasopharyngeal-carcinoma tissue samples and in 30 of 44 other head and neck tumor samples. HPV DNA sequence was detected in 14 of 30 nasopharyngeal-carcinoma tissue samples and in 11 of 44 other tumor samples. Coinfection of both viruses was observed in 14 nasopharyngeal-carcinoma tissue samples but only in 5 other head and neck tumor samples including 3 hypopharyngeal-carcinoma tissue samples. Our data indicate that EBV is closely associated with nasopharyngeal- carcinoma and may also be related to hypopharyngeal-carcinoma. In addition, a relatively high percentage of EBV-positive nasopharyngeal- and hypopharyngeal-carcinoma tissue specimens contained HPV sequence. The significance of the coexistence of EBV and HPV in these tumor tissues requires further study.
通过聚合酶链反应和DNA测序分析，检测了74例头颈部肿瘤组织中爱泼斯坦-巴尔病毒（EBV）DNA和人乳头瘤病毒（HPV）DNA的存在情况。在所有30份鼻咽癌组织样本以及44份其他头颈部肿瘤样本中的30份中检测到了EBV DNA序列。在30份鼻咽癌组织样本中的14份以及44份其他肿瘤样本中的11份中检测到了HPV DNA序列。在14份鼻咽癌组织样本中观察到两种病毒的共感染，但仅在包括3份下咽癌组织样本在内的5份其他头颈部肿瘤样本中观察到共感染。我们的数据表明，EBV与鼻咽癌密切相关，也可能与下咽癌有关。此外，EBV阳性的鼻咽癌和下咽癌组织标本中含有HPV序列的比例相对较高。EBV和HPV在这些肿瘤组织中共存的意义需要进一步研究。
