Furukawa Y, Nakatsuru S, Nagafuchi A, Tsukita S, Muto T, Nakamura Y, Horii A
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Cytogenet Cell Genet. 1994;65(1-2):74-8. doi: 10.1159/000133603.
We have isolated the human alpha-catenin gene (CTNNA1), which encodes a cadherin-associated protein, and have determined its primary structure and chromosomal localization. The transcript of CTNNA1 is 3.4 kb long and consists of 16 coding exons encoding 906 amino acids and at least one 5' noncoding exon. The 102-kDa predicted protein is the same size as the murine homolog, and the amino acid sequences of the two proteins are 99.2% homologous. Analysis by reverse transcription-PCR revealed that this gene is expressed ubiquitously in normal tissues. It was mapped to chromosome band 5q31 by fluorescent in situ hybridization.
我们分离出了人类α-连环蛋白基因（CTNNA1），该基因编码一种与钙黏着蛋白相关的蛋白质，并确定了其一级结构和染色体定位。CTNNA1的转录本长度为3.4 kb，由16个编码外显子组成，这些外显子编码906个氨基酸以及至少一个5'非编码外显子。预测的102 kDa蛋白质与小鼠同源物大小相同，两种蛋白质的氨基酸序列同源性为99.2%。逆转录聚合酶链反应分析表明，该基因在正常组织中普遍表达。通过荧光原位杂交将其定位到染色体5q31带。
