Shah S M, Spalton D J, Allen R J, Smith S E
Medical Eye Unit, St. Thomas' Hospital, London, England.
Invest Ophthalmol Vis Sci. 1993 Oct;34(11):3124-30.
To evaluate and compare the use of the Kowa laser flare cell meter and intravenous anterior chamber fluorophotometry in assessment of the blood-aqueous barrier after cataract surgery.
Laser flare and cell measurements and fluorophotometry were performed at 1 and 3 months after surgery in 48 eyes of 44 patients admitted for routine cataract surgery. The fellow pseudophakic eyes of these patients were used as controls.
The two techniques measure different parameters, but both methods are able to document the integrity or breakdown of the blood-aqueous barrier. However, the laser flare cell meter is more sensitive in quantifying subtle changes in barrier function to large molecules (proteins). Various methods of assessing anterior chamber fluorophotometry data were also compared. Measurement of a diffusion coefficient (requiring the measurement of plasma fluorescence) was not found to be more sensitive than other methods and did not alter the clinical significance of data obtained from the measurement of anterior chamber fluorescence alone.
Both the laser flare cell meter and fluorophotometry provide a method for the assessment of the postoperative blood-aqueous barrier. However, the laser flare cell meter is rapid, noninvasive, and relatively easier to use. Therefore, for clinical use, it has great practical advantages over fluorophotometry.
评估并比较科瓦激光闪光细胞仪和静脉注射前房荧光光度测定法在白内障手术后血-房水屏障评估中的应用。
对44例因常规白内障手术入院患者的48只眼在术后1个月和3个月进行激光闪光和细胞测量以及荧光光度测定。这些患者的对侧人工晶状体眼用作对照。
这两种技术测量的参数不同，但两种方法都能够记录血-房水屏障的完整性或破坏情况。然而，激光闪光细胞仪在量化屏障功能对大分子（蛋白质）的细微变化方面更敏感。还比较了评估前房荧光光度测定数据的各种方法。未发现测量扩散系数（需要测量血浆荧光）比其他方法更敏感，并且不会改变仅从前房荧光测量获得的数据的临床意义。
激光闪光细胞仪和荧光光度测定法都提供了一种评估术后血-房水屏障的方法。然而，激光闪光细胞仪快速、无创且相对易于使用。因此，对于临床应用，它比荧光光度测定法具有很大的实际优势。
