el-Harazi S M, Feldman R M, Chuang A Z, Ruiz R S, Villanueva G
Department of Ophthalmology and Visual Science, University of Texas Health Science Center, Houston, USA.
Ophthalmic Surg Lasers. 1998 May;29(5):380-4.
To determine the interobserver and intraobserver reproducibility of the laser flare meter and laser cell counter in assessing anterior chamber inflammation following cataract surgery.
Thirty-nine eyes with varied degrees of inflammation and 9 normal eyes were included in the study. Anterior chamber flare and cells were evaluated with the slit lamp and graded on a scale of 0 to 4+. The flare and cells were then measured three times each using the flare meter and cell counter by two different, experienced observers. The intraobserver reproducibilities were computed to evaluate repeatability of the instruments operated by the same observer. The first measurement taken by each observer was used to assess reproducibility between the two observers.
The intraobserver reproducibility for overall flare was 0.995 for both observers. The intraobserver reproducibility for overall cells was 0.996 for observer 1 and 0.991 for observer 2. The overall interobserver reproducibility was 0.994 for flare and 0.988 for cells. The correlation between measurements and slit-lamp ratings was 0.78 for flare and 0.56 for cells.
The intraobserver and interobserver reproducibilities were excellent, with good correlation to slit-lamp measurements. Reproducible results can be obtained using the laser flare meter and the laser cell counter. It is not known whether these results are applicable to other inflammatory processes with higher grades of flare and cells and with different protein and cell types.
确定激光散射仪和激光细胞计数仪在评估白内障手术后前房炎症时观察者间和观察者内的可重复性。
本研究纳入39只伴有不同程度炎症的眼睛和9只正常眼睛。使用裂隙灯评估前房炎症和细胞，并按0至4+级进行分级。然后，由两名经验丰富的不同观察者使用散射仪和细胞计数仪对炎症和细胞各测量三次。计算观察者内的可重复性，以评估同一观察者操作仪器的重复性。每位观察者的首次测量用于评估两名观察者之间的可重复性。
两名观察者的总体炎症散射的观察者内可重复性均为0.995。观察者1的总体细胞观察者内可重复性为0.996，观察者2为0.991。观察者间总体可重复性，炎症散射为0.994，细胞为0.988。测量结果与裂隙灯分级之间的相关性，炎症散射为0.78，细胞为0.56。
观察者内和观察者间的可重复性极佳，与裂隙灯测量结果具有良好的相关性。使用激光散射仪和激光细胞计数仪可获得可重复的结果。尚不清楚这些结果是否适用于其他具有更高炎症散射和细胞等级以及不同蛋白质和细胞类型的炎症过程。
