Zhou Q, Smith T L, Kummerow F A
Department of Food Science, University of Illinois, Urbana 61801.
Proc Soc Exp Biol Med. 1993 Jan;202(1):75-80. doi: 10.3181/00379727-202-43514.
The lethal effect of 25- and 26-hydroxycholesterol on smooth muscle cells derived from human umbilical arteries was investigated. The extent of cellular death corresponded with increasing oxysterol concentrations and incubation times. Incubation of the cells with 0.5, 2.5, or 10 micrograms/ml of 25- or 26-hydroxycholesterol revealed a proportionality between the degree of cellular death and oxysterol concentration over the 5 days of the experiment. Correlation coefficients among the degree of cellular death, the exposing period, and oxysterol concentration were significantly different (P < 0.05). However, none of these changes were noted in the smooth muscle cells cultured for 5 days in a medium containing the same concentration of cholesterol or 0.5% ethanol. An increase in the concentration of either serum or cholesterol in the culture medium did not significantly reduce the cytotoxicity of 2.5 micrograms/ml of 26-hydroxycholesterol. The results of this study suggest that oxysterols have an injurious effect on arterial cells, and that the injurious effect could not be altered by cholesterol, which was present at a serum concentration 12 times higher than that of oxysterols.
研究了25-羟基胆固醇和26-羟基胆固醇对人脐动脉平滑肌细胞的致死作用。细胞死亡程度与氧化甾醇浓度及孵育时间的增加相对应。用0.5、2.5或10微克/毫升的25-羟基胆固醇或26-羟基胆固醇孵育细胞，在实验的5天内，细胞死亡程度与氧化甾醇浓度之间呈现出比例关系。细胞死亡程度、暴露时间和氧化甾醇浓度之间的相关系数有显著差异（P<0.05）。然而，在含有相同浓度胆固醇或0.5%乙醇的培养基中培养5天的平滑肌细胞中，未观察到这些变化。培养基中血清或胆固醇浓度的增加并未显著降低2.5微克/毫升26-羟基胆固醇的细胞毒性。本研究结果表明，氧化甾醇对动脉细胞有损伤作用，且这种损伤作用不会因胆固醇而改变，胆固醇的血清浓度比氧化甾醇高12倍。
