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嗜热蓝细菌绿胶藻HTF(“层理鞭枝藻HTF”)菌株PCC7518的16S核糖体RNA结构及系统发育分析。

Structure of the 16 S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF ('Mastigocladus laminosus HTF') strain PCC7518, and phylogenetic analysis.

作者信息

Wilmotte A, Van der Auwera G, De Wachter R

机构信息

Department Biochemie, Universiteit Antwerpen, (UIA), Belgium.

出版信息

FEBS Lett. 1993 Feb 8;317(1-2):96-100. doi: 10.1016/0014-5793(93)81499-p.

Abstract

The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were observed in the original isolate. Alternatively, contamination may have happened. To solve this problem, the 16 S rRNA sequence was determined and used to infer a secondary structure model and build distance trees. The trees showed that strain PCC7518 belongs to the cluster of heterocystous species and has most probably lost the ability to produce heterocysts by mutation. It is only distantly related to Chlorogloeopsis fritschii PCC6718.

摘要

嗜热蓝藻菌株PCC7518最初被鉴定为“层理鞭枝藻HTF”,它没有分支或异形胞。没有分支支持了后来将其归入绿胶藻属的分类。没有异形胞可能是突变的结果,因为在原始分离物中观察到了异形胞。或者,可能发生了污染。为了解决这个问题,测定了16S rRNA序列,并用于推断二级结构模型和构建距离树。这些树表明,菌株PCC7518属于异形胞物种的聚类,很可能已经通过突变失去了产生异形胞的能力。它与绿胶藻PCC6718的亲缘关系较远。

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