Miller W G, McKenney J M, Conner M R, Chinchilli V M
Department of Pathology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0597.
Clin Chem. 1993 Feb;39(2):297-304.
We report the accuracy, imprecision, total analytical errors, and patient misclassification errors for cholesterol measured from capillary whole blood, venous whole blood, and venous plasma samples by five devices used in public cholesterol screening environments: Reflotron, Vision, Ektachem DT-60, QuickRead, and Liposcan. None of the methods met the National Cholesterol Education Program (NCEP) performance recommendations of 3% CV with 3% bias. The Vision and Reflotron methods used with venous samples gave individual results with total errors consistent with a combined CV and bias in the 4-5% range; capillary blood samples had total errors > 5% (combined CV and bias criteria). The DT-60 performance was near the 5% total error criterion for capillary samples and was > 5% for venous samples. Misclassification of individuals into desirable or referral groups for venous samples was as great as 5.1% for the DT-60, 5.7% for the Vision, and 7.1% for the Reflotron. Misclassifications for capillary blood samples were as great as 6.7%, 18.3%, and 14.1% for DT-60, Vision, and Reflotron, respectively. The QuickRead and Liposcan results were substantially poorer than those obtained by the other methods.
我们报告了在公共胆固醇筛查环境中使用的五种设备（Reflotron、Vision、Ektachem DT - 60、QuickRead和Liposcan）对毛细血管全血、静脉全血和静脉血浆样本中胆固醇测量的准确性、不精密度、总分析误差和患者错误分类误差。没有一种方法达到国家胆固醇教育计划（NCEP）规定的3%变异系数（CV）和3%偏差的性能建议。使用静脉样本的Vision和Reflotron方法得出的个体结果的总误差与4 - 5%范围内的综合CV和偏差一致；毛细血管血样本的总误差>5%（综合CV和偏差标准）。DT - 60对毛细血管样本的性能接近5%的总误差标准，对静脉样本则>5%。对于静脉样本，DT - 60将个体误分类到理想或转诊组的比例高达5.1%，Vision为5.7%，Reflotron为7.1%。DT - 60、Vision和Reflotron对毛细血管血样本的误分类分别高达6.7%、18.3%和14.1%。QuickRead和Liposcan的结果比其他方法获得的结果差得多。
