胰腺炎时肠道大分子物质通透性与大鼠疾病严重程度相关。

Gut macromolecular permeability in pancreatitis correlates with severity of disease in rats.

作者信息

Ryan C M, Schmidt J, Lewandrowski K, Compton C C, Rattner D W, Warshaw A L, Tompkins R G

机构信息

Massachusetts General Hospital, Department of Surgery, Harvard Medical School, Boston.

出版信息

Gastroenterology. 1993 Mar;104(3):890-5. doi: 10.1016/0016-5085(93)91027-f.

DOI:10.1016/0016-5085(93)91027-f

PMID:8440440

Abstract

BACKGROUND

Increased intestinal macromolecular permeability could allow absorption of substances from the bowel into the systemic circulation and contribute to multiple organ system failure.

METHODS

Mild, intermediate, and severe grades of pancreatitis were induced in rats using intravenous caerulein and intraductal glycodeoxycholic acid. [14C]polyethylene glycol (molecular weight, 3350 daltons; 1.1 microCi/142 mg) was instilled into the distal duodenum. At 24 hours, the animals were killed, ascitic fluid was collected for trypsinogen activation peptide measurement, and pancreatic specimens were collected and scored for based on the degree of necrosis, inflammation, and hemorrhage.

RESULTS

Gut permeability to polyethylene glycol 3350 (PEG 3350) was increased in animals with early experimental pancreatitis (5.4% +/- 1.2%, n = 20) when compared with control animals (1.8% +/- 0.2%; n = 6) (P = 0.0005). Furthermore, intestinal macromolecular permeability to PEG 3350 correlated with severity of disease as predicted by the method of induction of pancreatitis (P = 0.0003), the histological findings (P = 0.0002), and total ascitic trypsinogen activation peptides content (P = 0.029).

CONCLUSIONS

Increased gut permeability in experimental pancreatitis can be correlated with pancreatitis severity.

摘要

背景

肠道大分子通透性增加可使肠道内物质吸收入体循环，进而导致多器官系统功能衰竭。

方法

采用静脉注射蛙皮素和经导管注入甘氨脱氧胆酸的方法，在大鼠中诱导出轻度、中度和重度胰腺炎。将[14C]聚乙二醇（分子量3350道尔顿；1.1微居里/142毫克）注入十二指肠远端。24小时后，处死动物，收集腹水用于检测胰蛋白酶原激活肽，收集胰腺标本并根据坏死、炎症和出血程度进行评分。

结果

与对照动物（1.8%±0.2%；n = 6）相比，早期实验性胰腺炎动物（5.4%±1.2%，n = 20）对聚乙二醇3350（PEG 3350）的肠道通透性增加（P = 0.0005）。此外，肠道对PEG 3350的大分子通透性与胰腺炎诱导方法预测的疾病严重程度（P = 0.0003）、组织学结果（P = 0.0002）以及腹水中胰蛋白酶原激活肽的总含量（P = 0.029）相关。