Säämänen A M, Tammi M, Kiviranta I, Helminen H J
Department of Anatomy, University of Kuopio, Finland.
Int J Sports Med. 1988 Apr;9(2):127-33. doi: 10.1055/s-2007-1024993.
Proteoglycans (PGs) and collagen were quantified in the knee articular cartilages of femoral and tibial medial condyles following 1-8 weeks of moderate running exercise of 4- to 6-month-old rabbits. The total content of PGs extractable with 4 mol/l guanidium chloride was elevated in the weight-bearing cartilage of the tibial medial condyle, while their concentration, expressed as uronic acid per wet weight, and collagen remained unchanged. The content of glucosamine (GlcN) and its ratio to galactosamine (GalN) was elevated in femoral cartilage PGs purified by centrifugation in dissociative CsCl gradients, indicating an increase in keratan sulfate. After 8 weeks of running, the chondroitin sulfate chains of PGs from tibial medial condyle contained less unsulfated disaccharide units. The content of chondroitin sulfate was elevated in the nonextractable residue of the tibial medial condyle as indicated by uronic acid and GaN assays. The content of nonextractable GlcN was increased even more, both in tibial medial and femoral cartilages. Moderate running thus increased (1) keratan sulfate-rich PGs, (2) the degree of sulfation of the chondroitin sulfate chains, and (3) nonextractable PGs; these modulatory alterations probably enhance the stability and elastic stiffness of the PG matrix.
对4至6个月大的兔子进行为期1至8周的适度跑步锻炼后，对其股骨和胫骨内侧髁的膝关节软骨中的蛋白聚糖（PGs）和胶原蛋白进行了定量分析。用4 mol/l氯化胍可提取的PGs总含量在胫骨内侧髁的负重软骨中升高，而其浓度（以每湿重的糖醛酸表示）和胶原蛋白保持不变。通过在解离性CsCl梯度中离心纯化的股骨软骨PGs中，氨基葡萄糖（GlcN）的含量及其与半乳糖胺（GalN）的比率升高，表明硫酸角质素增加。跑步8周后，胫骨内侧髁PGs的硫酸软骨素链所含的未硫酸化二糖单位减少。通过糖醛酸和GaN测定表明，胫骨内侧髁不可提取残渣中硫酸软骨素的含量升高。不可提取的GlcN含量在胫骨内侧和股骨软骨中均增加得更多。因此，适度跑步增加了（1）富含硫酸角质素的PGs，（2）硫酸软骨素链的硫酸化程度，以及（3）不可提取的PGs；这些调节性改变可能增强了PG基质的稳定性和弹性硬度。
