Storch G A, Hall C B, Anderson L J, Park C S, Dohner D E
Edwin Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, St. Louis, MO 63110.
J Infect Dis. 1993 Mar;167(3):562-6. doi: 10.1093/infdis/167.3.562.
Monoclonal antibodies and ribonuclease protection were used to analyze antigenic and genomic diversity among 42 isolates of group A respiratory syncytial virus (RSV) from studies of nosocomial RSV carried out at the University of Rochester during the 1974-1975 and 1975-1976 RSV seasons. Three distinct subgroups or lineages and a total of 12 viral variants were present. Against this background of diversity, an outbreak was recognized that included 13 indistinguishable isolates occurring during a 2-week period. This outbreak accounted for 6 of the 8 infants with nosocomial infection. In contrast to the limited diversity of the nosocomial isolates, isolates from the 10 infants with community-acquired infection included 8 variants. Like those from community outbreaks of RSV, isolates of RSV from hospitalized patients are virologically heterogeneous. However, discrete outbreaks associated with transmission of a single strain can occur.
利用单克隆抗体和核糖核酸酶保护分析法，对1974 - 1975年和1975 - 1976年呼吸道合胞病毒（RSV）流行季期间，罗切斯特大学开展的医院内RSV研究中分离出的42株A组呼吸道合胞病毒（RSV）的抗原性和基因组多样性进行了分析。结果发现了三个不同的亚组或谱系，总共12种病毒变体。在这种多样性背景下，确认发生了一次暴发，其中包括在两周内出现的13株无法区分的分离株。此次暴发占8例医院感染婴儿中的6例。与医院内分离株有限的多样性形成对比的是，来自10例社区获得性感染婴儿的分离株包括8种变体。与RSV社区暴发的分离株一样，住院患者的RSV分离株在病毒学上具有异质性。然而，也可能发生与单一毒株传播相关的离散性暴发。
