Kauma S W
Department of Obstetrics/Gynecology, Medical College of Virginia, Richmond 23298.
J Clin Endocrinol Metab. 1993 Mar;76(3):701-3. doi: 10.1210/jcem.76.3.8445029.
The human placenta produces hematopoietic growth factors including colony stimulating factor-1 (CSF-1). We have previously demonstrated Interleukin-1 beta (IL-1 beta) production by decidualized endometrium during pregnancy. Since IL-1 beta stimulates CSF-1 production in a variety of mesenchymal cell types including second trimester villous core mesenchymal cells, the present study was designed to determine if IL-1 beta could also regulate CSF-1 production in term placental explants in vitro. Placental villous explants from normal term placentas (n = 5) were cultured with or without recombinant human IL-1 beta. A dose response relationship between increased added IL-1 beta and increased CSF-1 production as measured by enzyme-linked immunosorbent assay was observed with 1 ng/mL IL-1 beta being the lowest dose to significantly increase CSF-1 production (P < 0.01). In time course experiments, 10 ng/mL maximally induced CSF-1 messenger RNA expression 2.7 fold (P < 0.005) compared to controls at 8 h of culture as determined by dot blot analysis. Production rates of CSF-1 were linear up to 24 h at which time IL-1 beta (10 ng/mL)-treated samples had 1.7-fold higher levels of CSF-1 in the media than nontreated controls (8.38 ng/gm tissue vs. 4.94 ng/gm tissue, P < 0.01). These results demonstrate that IL-1 beta can regulate placental CSF-1 production in vitro and suggest that maternal decidual IL-1 beta may regulate placental CSF-1 production in vivo.
人类胎盘会产生包括集落刺激因子-1（CSF-1）在内的造血生长因子。我们之前已经证明，妊娠期间蜕膜化的子宫内膜会产生白细胞介素-1β（IL-1β）。由于IL-1β能刺激多种间充质细胞类型产生CSF-1，包括孕中期绒毛核心间充质细胞，因此本研究旨在确定IL-1β是否也能在体外调节足月胎盘外植体中CSF-1的产生。将来自正常足月胎盘的胎盘绒毛外植体（n = 5）在有或无重组人IL-1β的情况下进行培养。通过酶联免疫吸附测定法观察到，添加的IL-1β增加与CSF-1产生增加之间存在剂量反应关系，1 ng/mL的IL-1β是能显著增加CSF-1产生的最低剂量（P < 0.01）。在时间进程实验中，通过斑点印迹分析确定，与培养8小时的对照组相比，10 ng/mL的IL-1β在最大程度上诱导CSF-1信使核糖核酸表达增加了2.7倍（P < 0.005）。CSF-1的产生速率在24小时内呈线性，此时用IL-1β（10 ng/mL）处理的样本培养基中CSF-1的水平比未处理的对照组高1.7倍（8.38 ng/gm组织对4.94 ng/gm组织，P < 0.01）。这些结果表明，IL-1β可以在体外调节胎盘CSF-1的产生，并提示母体蜕膜IL-1β可能在体内调节胎盘CSF-1的产生。
