以内皮细胞腺嘌呤摄取作为细胞介导或补体介导细胞毒性的检测方法。
Endothelial adenine uptake as an assay for cell- or complement-mediated cytotoxicity.
作者信息
DeBono D P, Macintyre D E, White D J, Gordon J L
出版信息
Immunology. 1977 Feb;32(2):221-6.
Abstract
[3H] Adenine uptake by monolayers of cultured pig aortic endothelium was characterized and used as the basis for a terminal adenine uptake cytotoxicity assay. This assay was used to demonstrate specific cytotoxicity against donor-type endothelium by serum and by peripheral blood lymphocytes from skin-graft allergized pigs, and to compare the time course of these two types of effector mechanism.
摘要
用培养的猪主动脉内皮细胞单层对[3H]腺嘌呤的摄取进行了表征，并将其作为终末腺嘌呤摄取细胞毒性测定的基础。该测定用于证明血清和来自皮肤移植致敏猪的外周血淋巴细胞对供体类型内皮细胞的特异性细胞毒性，并比较这两种效应机制的时间进程。
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