Yamasaki H, Huberman E, Sachs L
Int J Cancer. 1977 Mar 15;19(3):378-82. doi: 10.1002/ijc.2910190315.
Aryl hydrocarbon (benzo(a)pyrene) hydroxylase (AHH) activity and metabolism of benzo(a)pyrene to water-soluble products were measured in cultures of body fibroblasts and kidney epithelial cells from different human embryos. AHH activity at 24 h after treatment with or without benz(a)anthracene was determined in cultures from 23 embryos, and 3 days' accumulated metabolism of benzo(a)pyrene to water soluble products was measured in cultures from 18 embryos. The body fibroblasts from the different embryos could be divided into three groups according to the amount of water-soluble products, but not according to the AHH activity. These three groups were not found by either assay in the cultures of kidney epithelial cells. In both fibroblast and epithelial cells, high metabolism to water-soluble products was not necessarily associated with high AHH activity. The results extend our previous finding (Huberman and Sachs, 1973) of three presumably genetic groups for BP metabolism to water-soluble products in human fibroblast but not in epithelial cells and indicate that this grouping was not found in these cells by measuring AHH activity.
在来自不同人类胚胎的人体成纤维细胞和肾上皮细胞培养物中，测定了芳烃（苯并(a)芘）羟化酶（AHH）活性以及苯并(a)芘向水溶性产物的代谢情况。在用或不用苯并(a)蒽处理24小时后，测定了来自23个胚胎的培养物中的AHH活性，并在来自18个胚胎的培养物中测量了苯并(a)芘向水溶性产物的3天累积代谢情况。不同胚胎的人体成纤维细胞可根据水溶性产物的量分为三组，但不能根据AHH活性分组。在肾上皮细胞培养物的两种测定中均未发现这三组。在成纤维细胞和上皮细胞中，向水溶性产物的高代谢不一定与高AHH活性相关。这些结果扩展了我们之前（Huberman和Sachs，1973年）的发现，即在人类成纤维细胞而非上皮细胞中，苯并芘向水溶性产物的代谢可能存在三个遗传组，并且表明通过测量AHH活性在这些细胞中未发现这种分组。
