Shimoya K, Matsuzaki N, Tsutsui T, Taniguchi T, Saji F, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Fertil Steril. 1993 Apr;59(4):885-8.
To determine if interleukin-8 (IL-8) is a normal constituent of seminal plasma and if leukospermia is a factor determining its elevation.
Seminal plasma from 58 men obtained by masturbation was examined for the presence of IL-8 using an IL-8 specific sandwich ELISA. Semen samples were obtained from 34 infertile men without leukospermia, 10 infertile men with leukospermia, and 14 proven fertile men. The correlation of amount of IL-8 in seminal plasma with some spermiogram parameters and the amount of polymorphonuclear (PMN) elastase was statistically evaluated.
Immunoreactive IL-8 was observed in the seminal plasma of all 58 subjects. The IL-8 titer in seminal plasma of patients with leukospermia (6.16 +/- 0.82 micrograms/L) was significantly higher than that in seminal plasma of patients without leukospermia (2.35 +/- 0.34 micrograms/L) and fertile men (1.64 +/- 0.29 micrograms/L). There was a high degree of correlation between PMN elastase and IL-8 levels in seminal plasma.
These findings demonstrate IL-8 to be in seminal plasma and elevated IL-8 levels in infertile patients with leukospermia.
确定白细胞介素-8（IL-8）是否为精浆的正常成分，以及白细胞增多症是否是决定其升高的一个因素。
采用IL-8特异性夹心酶联免疫吸附测定法（ELISA），检测58名通过手淫获取精液的男性的精浆中IL-8的存在情况。精液样本取自34名无白细胞增多症的不育男性、10名有白细胞增多症的不育男性以及14名已证实有生育能力的男性。对精浆中IL-8含量与一些精液参数以及多形核（PMN）弹性蛋白酶含量之间的相关性进行统计学评估。
在所有58名受试者的精浆中均观察到免疫反应性IL-8。有白细胞增多症患者的精浆中IL-8滴度（6.16±0.82微克/升）显著高于无白细胞增多症患者的精浆（2.35±0.34微克/升）和有生育能力男性的精浆（1.64±0.29微克/升）。精浆中PMN弹性蛋白酶与IL-8水平之间存在高度相关性。
这些发现表明IL-8存在于精浆中，且在有白细胞增多症的不育患者中IL-8水平升高。
