使用紫外线平行透镜适配器替代传统荧光显微镜，用于在直接免疫荧光单克隆抗体染色的肺部标本中检测卡氏肺孢子虫。
Use of the UV ParaLens adapter as an alternative to conventional fluorescence microscopy for detection of Pneumocystis carinii in direct immunofluorescent monoclonal antibody-stained pulmonary specimens.
作者信息
Mattia A R, Waldron M A, Sierra L S
机构信息
Department of Pathology, Massachusetts General Hospital, Boston 02114.
出版信息
J Clin Microbiol. 1993 Mar;31(3):720-1. doi: 10.1128/jcm.31.3.720-721.1993.
Abstract
The UV ParaLens light microscope adapter offers a useful and cost-effective alternative to conventional fluorescence microscopy for Pneumocystis carinii identification, particularly in AIDS patients. In a blinded study, in which 153 pulmonary specimens were examined for P. carinii by direct immunofluorescence, 40 of 42 specimens positive by fluorescence microscopy were also positive by ParaLens. No false positives were observed.
摘要
紫外线平行透镜光学显微镜适配器为卡氏肺孢子虫的鉴定提供了一种实用且经济高效的替代传统荧光显微镜检查的方法，尤其适用于艾滋病患者。在一项盲法研究中，通过直接免疫荧光对153份肺部标本进行卡氏肺孢子虫检测，荧光显微镜检查呈阳性的42份标本中有40份通过平行透镜检查也呈阳性。未观察到假阳性结果。
相似文献
1
Use of the UV ParaLens adapter as an alternative to conventional fluorescence microscopy for detection of Pneumocystis carinii in direct immunofluorescent monoclonal antibody-stained pulmonary specimens.使用紫外线平行透镜适配器替代传统荧光显微镜，用于在直接免疫荧光单克隆抗体染色的肺部标本中检测卡氏肺孢子虫。
J Clin Microbiol. 1993 Mar;31(3):720-1. doi: 10.1128/jcm.31.3.720-721.1993.
2
Detection of Pneumocystis carinii by fluorescent-antibody stain using a combination of three monoclonal antibodies.使用三种单克隆抗体组合通过荧光抗体染色检测卡氏肺孢子虫。
J Clin Microbiol. 1987 Oct;25(10):1837-40. doi: 10.1128/jcm.25.10.1837-1840.1987.
3
Pneumocystis carinii pneumonia: detection of parasites by immunofluorescence based on a monoclonal antibody.卡氏肺孢子虫肺炎：基于单克隆抗体通过免疫荧光法检测寄生虫。
Med Lab Sci. 1991 Jul;48(3):235-7.
4
Blinded comparison of a direct immunofluorescent monoclonal antibody staining method and a Giemsa staining method for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens of patients infected with human immunodeficiency virus.在感染人类免疫缺陷病毒患者的诱导痰和支气管肺泡灌洗标本中，采用直接免疫荧光单克隆抗体染色法与吉姆萨染色法对卡氏肺孢子虫进行鉴定的盲法比较。
J Clin Microbiol. 1990 Sep;28(9):2136-8. doi: 10.1128/jcm.28.9.2136-2138.1990.
5
Rapid detection of Pneumocystis carinii using a direct fluorescent monoclonal antibody stain.使用直接荧光单克隆抗体染色法快速检测卡氏肺孢子虫。
J Clin Microbiol. 1990 Oct;28(10):2228-33. doi: 10.1128/jcm.28.10.2228-2233.1990.
6
Improved detection of Pneumocystis carinii by an immunofluorescence technique using monoclonal antibodies.采用单克隆抗体免疫荧光技术改进卡氏肺孢子虫的检测。
Eur J Clin Microbiol Infect Dis. 1990 Dec;9(12):880-5. doi: 10.1007/BF01967503.
7
8
Cytologic detection of Pneumocystis carinii: a comparison of Papanicolaou and other histochemical stains.
Diagn Cytopathol. 1986 Apr-Jun;2(2):133-7. doi: 10.1002/dc.2840020207.
9
Detection of Pneumocystis carinii with direct fluorescence antibody and calcofluor white stain.用直接荧光抗体和荧光增白剂染色法检测卡氏肺孢子虫。
Infection. 1996 May-Jun;24(3):248-50. doi: 10.1007/BF01781104.
10
Laboratory diagnosis and occurrence of Pneumocystis carinii.卡氏肺孢子虫的实验室诊断与发病情况
Scand J Infect Dis Suppl. 1994;94:1-34.
引用本文的文献
1
Tackling HIV through robust diagnostics in the developing world: current status and future opportunities.通过在发展中国家加强诊断技术来应对艾滋病毒：现状与未来机遇。
Lab Chip. 2011 Jan 21;11(2):194-211. doi: 10.1039/c0lc00340a. Epub 2010 Dec 1.
2
Usefulness of the paralens fluorescent microscope adaptor for the identification of mycobacteria in both field and laboratory settings.Paralens荧光显微镜适配器在现场和实验室环境中对分枝杆菌鉴定的实用性。
Open Microbiol J. 2010 Apr 30;4:30-3. doi: 10.2174/1874285801004010030.
3
Use of the Quantitative Buffy Coat system for detection of parasitemia in patients with babesiosis.使用定量血沉棕黄层系统检测巴贝斯虫病患者的寄生虫血症。
J Clin Microbiol. 1993 Oct;31(10):2816-8. doi: 10.1128/jcm.31.10.2816-2818.1993.
4
Prospective comparison of direct immunofluorescence and conventional staining methods for detection of Giardia and Cryptosporidium spp. in human fecal specimens.直接免疫荧光法与传统染色法检测人体粪便标本中贾第虫和隐孢子虫属的前瞻性比较
J Clin Microbiol. 1995 Jun;33(6):1632-4. doi: 10.1128/jcm.33.6.1632-1634.1995.
5
Use of UV ParaLens adapter for detection of acid-fast organisms.使用紫外线副透镜适配器检测抗酸菌。
J Clin Microbiol. 1995 Jan;33(1):239-41. doi: 10.1128/jcm.33.1.239-241.1995.
本文引用的文献
1
Advances in pneumocystis biology.肺囊虫生物学进展
Parasitol Today. 1986 May;2(5):137-42. doi: 10.1016/0169-4758(86)90178-x.
2
The usefulness of induced sputum in the diagnosis of Pneumocystis carinii pneumonia in patients with the acquired immunodeficiency syndrome.诱导痰在获得性免疫缺陷综合征患者卡氏肺孢子虫肺炎诊断中的应用价值。
Am Rev Respir Dis. 1986 Apr;133(4):515-8. doi: 10.1164/arrd.1986.133.4.515.
3
Comparison of methenamine silver nitrate and Giemsa stain for detection of Pneumocystis carinii in bronchoalveolar lavage specimens from HIV infected patients.硝酸银甲萘胺与吉姆萨染色法在检测HIV感染患者支气管肺泡灌洗标本中卡氏肺孢子虫的比较
APMIS. 1989 Aug;97(8):745-7. doi: 10.1111/j.1699-0463.1989.tb00472.x.
4
Corticosteroids as adjunctive therapy for severe Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome. A double-blind, placebo-controlled trial.皮质类固醇作为获得性免疫缺陷综合征中重度卡氏肺孢子虫肺炎的辅助治疗。一项双盲、安慰剂对照试验。
N Engl J Med. 1990 Nov 22;323(21):1444-50. doi: 10.1056/NEJM199011223232103.
5
Recent advances in the diagnosis, treatment, and prevention of Pneumocystis carinii pneumonia.卡氏肺孢子虫肺炎诊断、治疗及预防的最新进展
Antimicrob Agents Chemother. 1990 Apr;34(4):499-504. doi: 10.1128/AAC.34.4.499.
6
Pneumocystis carinii, an opportunist in immunocompromised patients.卡氏肺孢子菌，一种免疫功能低下患者体内的机会致病菌。
Clin Microbiol Rev. 1991 Apr;4(2):137-49. doi: 10.1128/CMR.4.2.137.
7
Laboratory diagnosis of pneumocystosis.
Clin Lab Med. 1991 Dec;11(4):957-75.
8
Blinded comparison of a direct immunofluorescent monoclonal antibody staining method and a Giemsa staining method for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens of patients infected with human immunodeficiency virus.在感染人类免疫缺陷病毒患者的诱导痰和支气管肺泡灌洗标本中，采用直接免疫荧光单克隆抗体染色法与吉姆萨染色法对卡氏肺孢子虫进行鉴定的盲法比较。
J Clin Microbiol. 1990 Sep;28(9):2136-8. doi: 10.1128/jcm.28.9.2136-2138.1990.
9
Rapid diagnosis of malaria with the QBC system in a hospital in Berlin, Germany.
Trans R Soc Trop Med Hyg. 1992 Jan-Feb;86(1):6. doi: 10.1016/0035-9203(92)90413-7.
10
Using the UV ParaLens adapter on a standard laboratory microscope for fluorescent rabies antibody detection.
Trans R Soc Trop Med Hyg. 1992 Jan-Feb;86(1):107. doi: 10.1016/0035-9203(92)90464-n.
Zotero 插件