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使用紫外线副透镜适配器检测抗酸菌。

Use of UV ParaLens adapter for detection of acid-fast organisms.

作者信息

Patterson K V, McDonald C L, Miller B F, Chapin K C

机构信息

Department of Pathology, University of South Alabama, Mobile 36617.

出版信息

J Clin Microbiol. 1995 Jan;33(1):239-41. doi: 10.1128/jcm.33.1.239-241.1995.

Abstract

Auramine-stained mycobacterial smears from 136 clinical specimens were interpreted by using the UV ParaLens adapter (Beckton Dickinson), and results were compared with smear interpretations using a traditional fluorescent microscope and culture. The sensitivity and specificity of the ParaLens were 84 and 93%, respectively. Smears yielding discrepant results were overstained by the Kinyoun method. Overall, the sensitivity of auramine-stained smears interpreted with the UV ParaLens was comparable to that of Kinyoun-stained smears.

摘要

使用紫外线ParaLens适配器(贝克顿·迪金森公司)对136份临床标本的金胺染色分枝杆菌涂片进行解读,并将结果与使用传统荧光显微镜和培养法的涂片解读结果进行比较。ParaLens的敏感性和特异性分别为84%和93%。产生不一致结果的涂片用金扬法过度染色。总体而言,用紫外线ParaLens解读的金胺染色涂片的敏感性与金扬染色涂片相当。

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