Selivonchick D P, Roots B I
Lipids. 1977 Feb;12(2):165-9. doi: 10.1007/BF02533288.
A method is described for the isolation of pure capillary endothelia from rat brain and the phosphlipid composition of these cells is reported. This method is rapid and requires only a small amount of starting material. It involves: (a) tissue disruption by high speed homogenization, (b) separation of the capillary endothelia from other brain structures using sucrose gradients, and (c) a final purification using a glass bead column. Choline and ethanolamine phosphoglycerides were found to be the predominant lipid classes of these cells amounting to 31.9% and 24.4%, respectively, of total phospholipids. The choline phosphoglycerides consisted almost exclusively of 1,2-diacyl glycerophosphorylcholine, whereas the ethanolamine phosphoglycerides consisted of approximately equal amounts of 1,2-diacyl and 1-alk-1'-enyl-2-acyl glycerophosphorylethanolamine. The composition of the constituent fatty acids of both choline and ethanolamine phosphoglycerides and the alk-1-enyl composition of ethanolamine phosphoglycerides is reported. Saturated fatty acids accounted for 45% of the total fatty acids in choline phosphoglycerides and for 53% in ethanolamine phosphoglycerides. Arachidonic acid accounted for approximately 48% of the total fatty acids in alk-1-enyl ethanolamine phosphoglyceride.
本文描述了一种从大鼠脑中分离纯毛细血管内皮细胞的方法，并报告了这些细胞的磷脂组成。该方法快速，仅需少量起始材料。它包括：（a）通过高速匀浆破坏组织，（b）使用蔗糖梯度从其他脑结构中分离毛细血管内皮细胞，以及（c）使用玻璃珠柱进行最终纯化。发现胆碱和乙醇胺磷酸甘油酯是这些细胞的主要脂质类别，分别占总磷脂的31.9%和24.4%。胆碱磷酸甘油酯几乎完全由1,2 - 二酰基甘油磷酸胆碱组成，而乙醇胺磷酸甘油酯由大约等量的1,2 - 二酰基和1 - 烯基 - 2 - 酰基甘油磷酸乙醇胺组成。报告了胆碱和乙醇胺磷酸甘油酯的组成脂肪酸以及乙醇胺磷酸甘油酯的烯基组成。饱和脂肪酸在胆碱磷酸甘油酯的总脂肪酸中占45%，在乙醇胺磷酸甘油酯中占53%。花生四烯酸在烯基乙醇胺磷酸甘油酯的总脂肪酸中约占48%。
