Cook E H, Perry B D, Dawson G, Wainwright M S, Leventhal B L
Department of Psychiatry, University of Chicago.
J Autism Dev Disord. 1993 Mar;23(1):67-78. doi: 10.1007/BF01066419.
Forty-two parents of children with autistic disorder, 15 children with autistic disorder, 17 siblings of children with autistic disorder, and 12 unrelated normal adult controls were studied to determine if immunoglobulins isolated from their plasma would inhibit binding of the 5HT1A agonist, [3H]-8-hydroxy-N,N-dipropyl-2-aminotetralin (DPAT) to 5HT1A receptors in human hippocampal membranes. There were no significant differences among the means of percentage inhibition of DPAT binding of parents, children with autistic disorder, siblings, or unrelated controls. In addition, there were no differences in the proportion of subjects with > 15% DPAT inhibition among autistic children, their parents, their siblings, or unrelated controls. Immunoglobulin inhibition was not specific for the 5HT1A receptor binding site, since immunoglobulins inhibited binding to 5HT2, D1, D2, and alpha 2-adrenergic binding sites. The immunoglobulins isolated from normal controls inhibited [3H]-rauwolscine binding at alpha 2-adrenergic sites less than immunoglobulins of children with autistic disorder and their parents and siblings. This study did not support the hypothesis that autoantibodies to 5HT1A or 5HT2 receptors are characteristic of autistic disorder.
对42名自闭症谱系障碍儿童的父母、15名自闭症谱系障碍儿童、17名自闭症谱系障碍儿童的兄弟姐妹以及12名无血缘关系的正常成年对照者进行了研究，以确定从他们血浆中分离出的免疫球蛋白是否会抑制5HT1A激动剂[3H]-8-羟基-N,N-二丙基-2-氨基四氢萘（DPAT）与人脑海马膜中5HT1A受体的结合。在父母、自闭症谱系障碍儿童、兄弟姐妹或无血缘关系的对照者中，DPAT结合抑制百分比的平均值之间没有显著差异。此外，在自闭症儿童、其父母、其兄弟姐妹或无血缘关系的对照者中，DPAT抑制率>15%的受试者比例也没有差异。免疫球蛋白抑制并非特异性针对5HT1A受体结合位点，因为免疫球蛋白会抑制与5HT2、D1、D2和α2-肾上腺素能结合位点的结合。从正常对照者中分离出的免疫球蛋白对α2-肾上腺素能位点的[3H]-萝芙木碱结合的抑制作用小于自闭症谱系障碍儿童及其父母和兄弟姐妹的免疫球蛋白。这项研究不支持5HT1A或5HT2受体自身抗体是自闭症谱系障碍特征的假说。
