Holmes B, Moss C W, Daneshvar M I
Identification Services Laboratory, Central Public Health Laboratory, Colindale, London, United Kingdom.
J Clin Microbiol. 1993 Apr;31(4):1007-8. doi: 10.1128/jcm.31.4.1007-1008.1993.
Strains of "Achromobacter groups B and E" were examined for cellular fatty acid (CFA) composition to evaluate their chemical relatedness to known bacterial species and groups. The CFAs were liberated from whole cells by base hydrolysis, methylated, and analyzed by capillary gas-liquid chromatography. The CFA profiles of the two groups were identical and were distinct from CFA profiles of all other bacteria we have previously tested. These data provide support for results from whole-cell protein pattern analysis and DNA-DNA and rRNA-DNA hybridization studies, which show that "Achromobacter groups B and E" are biotypes of a single new genus and species.
对“无色杆菌B组和E组”菌株进行了细胞脂肪酸(CFA)组成分析,以评估它们与已知细菌种类和菌群的化学相关性。通过碱水解从全细胞中释放出CFA,进行甲基化处理,然后用毛细管气液色谱法进行分析。这两组的CFA图谱相同,且与我们之前测试过的所有其他细菌的CFA图谱不同。这些数据为全细胞蛋白质模式分析、DNA-DNA和rRNA-DNA杂交研究的结果提供了支持,这些研究表明“无色杆菌B组和E组”是一个单一新属和新物种的生物型。