通过亚磷酰胺法大规模液相合成寡核苷酸。
Large scale, liquid phase synthesis of oligonucleotides by the phosphoramidite approach.
作者信息
Bonora G M, Biancotto G, Maffini M, Scremin C L
机构信息
Department of Organic Chemistry, CNR, University of Padova, Italy.
出版信息
Nucleic Acids Res. 1993 Mar 11;21(5):1213-7. doi: 10.1093/nar/21.5.1213.
Abstract
A new method for the liquid phase synthesis of oligonucleotides is described which makes use of polyethylene glycol (PEG) as soluble support and phosphoramidite derivatives as synthons. The new synthetic protocol was applied to a quite large scale production (about 100 mumoles) of such compounds up to the 20mer level. This solution method, called HELP High Efficiency Liquid Phase) Plus, appears effective in terms of speed and coupling yield and can be evaluated for the production of large amount of oligonucleotides.
摘要
本文描述了一种寡核苷酸液相合成的新方法，该方法利用聚乙二醇（PEG）作为可溶性载体，亚磷酰胺衍生物作为合成子。这种新的合成方案已应用于大规模生产（约100微摩尔）此类化合物，直至20聚体水平。这种溶液法称为HELP（高效液相）Plus，在速度和偶联产率方面似乎很有效，可用于大量寡核苷酸的生产评估。
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