HELP（高效液相）在可溶性聚合物载体上的新型寡核苷酸合成。
HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support.
作者信息
Bonora G M, Scremin C L, Colonna F P, Garbesi A
机构信息
Department of Organic Chemistry, University of Padova, Italy.
出版信息
Nucleic Acids Res. 1990 Jun 11;18(11):3155-9. doi: 10.1093/nar/18.11.3155.
Abstract
A simple, rapid and high-yielding method for the synthesis of oligonucleotides by the phosphotriesters approach is described. The use of polyethylene glycol (PEG) as soluble polymeric support preserves some convenient features of the solid-phase synthesis with new interesting advantages. Short oligonucleotides in hundred milligrams scale can be obtained from few grams of functionalized PEG.
摘要
描述了一种通过磷酸三酯法合成寡核苷酸的简单、快速且高产的方法。使用聚乙二醇（PEG）作为可溶性聚合物载体保留了固相合成的一些便利特性，并具有新的有趣优势。从几克功能化PEG中可获得毫克级的短寡核苷酸。
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