Glencross R G, Lovell R D, Holder A T
Department of Biochemistry and Physiology, University of Reading, Whiteknights, U.K.
J Endocrinol. 1993 Mar;136(3):R5-7. doi: 10.1677/joe.0.136r005.
The enhancing effect of bovine FSH monoclonal antibody (bFSH-MAb) on the gonadotrophic activity of FSH was investigated in dwarf mice using a uterine weight bioassay. Increasing doses of bovine FSH (NIH-FSH-B1; 3.3, 10 or 30 micrograms/day) were administered for 5 days to dwarf mice (groups of five) with or without administration of a bFSH-MAb preparation (USDA-bFSH-MC28; 100 micrograms protein/day) which at a dilution of 1:15,000 bound 50% of 125I-labelled bFSH (USDA-bFSH-BP3). The bFSH, at the doses given, gave no increases in uterine weight; when, however, these doses were given with bFSH-MAb, significant (three- to four fold) increases in uterine weight resulted.
使用子宫重量生物测定法，在侏儒小鼠中研究了牛促卵泡素单克隆抗体（bFSH-MAb）对促卵泡素促性腺活性的增强作用。向五组侏儒小鼠连续5天给予递增剂量的牛促卵泡素（NIH-FSH-B1；3.3、10或30微克/天），其中一组同时给予bFSH-MAb制剂（USDA-bFSH-MC28；100微克蛋白质/天），该制剂在1:15,000稀释度下可结合50%的125I标记的bFSH（USDA-bFSH-BP3）。所给剂量的bFSH未使子宫重量增加；然而，当这些剂量与bFSH-MAb一起给予时，子宫重量显著增加（三到四倍）。
