Norman M E, Williams P, Illum L
Department of Pharmaceutical Sciences, University of Nottingham, UK.
Biomaterials. 1993 Feb;14(3):193-202. doi: 10.1016/0142-9612(93)90023-u.
SDS-PAGE in combination with densitometry has been used to evaluate the adsorption of plasma and serum proteins to polystyrene microspheres (PS) coated with block copolymers of the poloxamer and poloxamine series. The protein-resistant nature of coated PS was demonstrated for these systems when incubated in dilutions of plasma and serum. The total amount of protein and the type of proteins adsorbed were dependent on the plasma and serum incubation concentration used. At 0.3% serum concentrations the total amount of protein adsorbed was found to be related to the polyoxyethylene (PEO) chain length of the block copolymer, whilst at 0.3% or 50% plasma concentrations a relationship was shown between the polyoxypropylene (PPO) chain and the plasma protein adsorption for the range of block copolymers studied. Immunoblotting studies revealed the adsorption of immunoglobulin G, complement C3, transferrin and fibronectin to all microspheres previously incubated in 50% serum and plasma, whilst fibrinogen was also adsorbed after incubation in 50% plasma; with similar quantities of each protein adsorbed to PS and block copolymer-coated PS.
十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）结合光密度测定法已用于评估血浆和血清蛋白对涂有泊洛沙姆和泊洛胺系列嵌段共聚物的聚苯乙烯微球（PS）的吸附情况。当在血浆和血清稀释液中孵育时，这些系统证明了涂覆PS的抗蛋白质性质。吸附的蛋白质总量和蛋白质类型取决于所使用的血浆和血清孵育浓度。在血清浓度为0.3%时，发现吸附的蛋白质总量与嵌段共聚物的聚氧乙烯（PEO）链长度有关，而在血浆浓度为0.3%或50%时，在所研究的嵌段共聚物范围内，聚氧丙烯（PPO）链与血浆蛋白吸附之间存在关系。免疫印迹研究表明，免疫球蛋白G、补体C3、转铁蛋白和纤连蛋白吸附到所有先前在50%血清和血浆中孵育的微球上，而纤维蛋白原在50%血浆中孵育后也被吸附；每种蛋白质吸附到PS和嵌段共聚物涂覆的PS上的量相似。
