Chaudhuri B, Sau S, Datta H J, Mandal N C
Department of Biochemistry, Bose Institute, Calcutta, India.
Virology. 1993 May;194(1):166-72. doi: 10.1006/viro.1993.1246.
Forty temperature-sensitive mutations affecting lytic growth and eight affecting both establishment and maintenance of lysogeny of the temperate mycobacteriophage L1 have been isolated. All of the latter mutations form one complementation group and map within a very short region around the 15% coordinate of the L1 genome; these affect a single gene, cl, coding for the L1 repressor. The former 40 mutations form 28 complementation groups, identifying 28 different genes, G1-G28, essential for the lytic growth of L1. These genes have been mapped using the Gts mutations. Of the 28 Gts mutants, 14 are defective in host lysis at 42 degrees but not at 32 degrees while the other 14 can lyse the host at both temperatures. Among the former 14 Gts mutants, 6 are also defective in L1 DNA synthesis at 42 degrees, and they map in two different clusters, 4 around 65% and 2 around 84% of the L1 genome.
已分离出40个影响裂解生长的温度敏感突变体和8个影响温和分枝杆菌噬菌体L1溶原性建立与维持的突变体。所有后一类突变体形成一个互补群，并定位在L1基因组15%坐标附近的一个非常短的区域内；这些突变影响一个单一基因cl，该基因编码L1阻遏物。前40个突变体形成28个互补群，鉴定出28个不同的基因G1 - G28，它们对L1的裂解生长至关重要。这些基因已通过Gts突变进行定位。在28个Gts突变体中，14个在42℃时宿主裂解有缺陷，但在32℃时无缺陷，而另外14个在两个温度下都能裂解宿主。在前14个Gts突变体中，6个在42℃时L1 DNA合成也有缺陷，它们定位在两个不同的簇中，4个在L1基因组的65%左右，2个在84%左右。
